Team:Groningen/Notebook/20 July 2009

From 2009.igem.org

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===Transporters===
===Transporters===
Below the PCR of 17/07, There seems to be a vague band at ~1000 in 1.  
Below the PCR of 17/07, There seems to be a vague band at ~1000 in 1.  
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 +
[[Image:PCR15-7-19.jpg]] [[Image:F102471_2009-07-17_05hr_30min_A_E,_small_band.jpg]]
[[Image:PCR15-7-19.jpg]] [[Image:F102471_2009-07-17_05hr_30min_A_E,_small_band.jpg]]
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|Denaturing
|Denaturing
-
|98°
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|95°
-
|1.30 min
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|30 sec
|-
|-
|
|
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|-
|-
|Denaturing
|Denaturing
-
|98°
+
|95°
-
|10 sec
+
|20 sec
|-
|-
|Annealing
|Annealing
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|Elongation
|Elongation
|72°
|72°
-
|30 sec
+
|1.10 min
|-
|-
|  
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Revision as of 15:13, 20 July 2009

Igemhomelogo.png

Wet

GVP Cluster

Transporters

Below the PCR of 17/07, There seems to be a vague band at ~1000 in 1.


PCR15-7-19.jpg F102471 2009-07-17 05hr 30min A E, small band.jpg


The band at ~1000 kb was cut out of the gel and used for pcr.

PCR1 gel
Component amount
MasterMix NH4 40 uL
HmtA Fw 1 uL
HmtA mut1RC 1 uL
PCR1 band
Taq polymerase 2uL
PCR1 program Temperature Time
Denaturing 95° 30 sec
Start Cycles 30X
Denaturing 95° 20 sec
Annealing 55° 20 sec
Elongation 72° 1.10 min
End cycles
Final elongation 72° 10 min
Hold Forever

Metal Accumulation

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30