Team:Groningen/Notebook/20 July 2009

From 2009.igem.org

(Difference between revisions)
(→Vectors)
(→Wet)
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[[image:F102471_2009-07-20_08hr_30min_noted.JPG]]
[[image:F102471_2009-07-20_08hr_30min_noted.JPG]]
 +
 +
<!--Tabel 1 hier-->
 +
 +
{| border="1"
 +
|+ '''VR-VF2'''
 +
! Component !! amount
 +
|-
 +
! MasterMix NH4
 +
| 21 uL
 +
|-
 +
! F primer
 +
| 1 uL
 +
|-
 +
! R primer
 +
| 1 uL
 +
|-
 +
! psB1AC3 vector
 +
| 0.5 uL
 +
|-
 +
! Taq polymerase
 +
| 1 uL
 +
|}
 +
<!--tabel 2 hier-->
 +
{|border="1"
 +
!Cup nr
 +
!F primer
 +
!R primer
 +
|-
 +
|1
 +
|VF2 iGEMGr09
 +
|VR iGEMGr09
 +
|-
 +
|2
 +
|VF2 iGEMGr09
 +
|VR iGEMGr08 Aug
 +
|-
 +
|3
 +
|VF2 iGEMGr09
 +
|VR iGEMGr June
 +
|-
 +
|4
 +
|VF2 iGEMGr08
 +
|VR iGEMGr09
 +
|-
 +
|5
 +
|VF2 iGEMGr08
 +
|VR iGEMGr08 Aug
 +
|-
 +
|6
 +
|VF2 iGEMGr08
 +
|VR iGEMGr June
 +
|}
 +
 +
 +
<!--Tabel 2 hier-->
 +
{|
 +
! PCR1 program
 +
! Temperature
 +
! Time
 +
|-
 +
|Denaturing
 +
|95°
 +
|5 min
 +
|-
 +
|
 +
|Start Cycles 30X
 +
|-
 +
|Denaturing
 +
|95°
 +
|20 sec
 +
|-
 +
|Annealing
 +
|55°
 +
|20 sec
 +
|-
 +
|Elongation
 +
|72°
 +
|1.10 min
 +
|-
 +
|
 +
|End cycles
 +
|-
 +
|Final elongation
 +
|72°
 +
|10 min
 +
|-
 +
|Hold
 +
|4°
 +
|Forever
 +
|}
==Dry==
==Dry==
{{Team:Groningen/Notebook/Day/Footer}}
{{Team:Groningen/Notebook/Day/Footer}}

Revision as of 15:54, 20 July 2009

Igemhomelogo.png

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Wet

GVP Cluster

Transporters

Below the PCR of 17/07, There seems to be a vague band at ~1150 in 1.


F102471 2009-07-17 08hr 29min pcr1,pcr2 noted.JPG

The band at ~1150 kb was cut out of the gel and used for pcr described below.

PCR1 gel
Component amount
MasterMix NH4 40 uL
HmtA Fw 1 uL
HmtA mut1RC 1 uL
PCR1 band
Taq polymerase 2uL


PCR1 program Temperature Time
Denaturing 95° 5 min
Start Cycles 30X
Denaturing 95° 20 sec
Annealing 55° 20 sec
Elongation 72° 1.10 min
End cycles
Final elongation 72° 10 min
Hold 4° Forever

Metal Accumulation

Vectors

positive control psB1AC3

F102471 2009-07-20 08hr 30min noted.JPG


VR-VF2
Component amount
MasterMix NH4 21 uL
F primer 1 uL
R primer 1 uL
psB1AC3 vector 0.5 uL
Taq polymerase 1 uL
Cup nr F primer R primer
1 VF2 iGEMGr09 VR iGEMGr09
2 VF2 iGEMGr09 VR iGEMGr08 Aug
3 VF2 iGEMGr09 VR iGEMGr June
4 VF2 iGEMGr08 VR iGEMGr09
5 VF2 iGEMGr08 VR iGEMGr08 Aug
6 VF2 iGEMGr08 VR iGEMGr June


PCR1 program Temperature Time
Denaturing 95° 5 min
Start Cycles 30X
Denaturing 95° 20 sec
Annealing 55° 20 sec
Elongation 72° 1.10 min
End cycles
Final elongation 72° 10 min
Hold 4° Forever

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
Retrieved from "http://2009.igem.org/Team:Groningen/Notebook/20_July_2009"