Team:Groningen/Notebook/22 September 2009
From 2009.igem.org
Wet
GVP Cluster
Several ON cultures did grow Several plates did grow
Transporters
Metal Accumulation
ICP-MS
ON starter cultures continued for creating biomass for As uptake experiments combined with promotor activation and methalotein binding. Use E. coli + control vector, E. coli + pArsR-RFP, E. coli + pLac-fMT Take OD600 samples every 1-1.5 hrs of E. coli + pLac-fMT. Induce E. coli + pLac-fMT at OD600 ~0.6 with 0.5mM IPTG.
Vectors
Dry
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