Team:Groningen/Notebook/27 July 2009
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===Metal Accumulation=== | ===Metal Accumulation=== | ||
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- | Gel with ArsR PCR | + | |
+ | Gel with ArsR PCR((24/07/09) | ||
[[Image:270709_ArsR+GlpF.JPG]] | [[Image:270709_ArsR+GlpF.JPG]] | ||
+ | |||
+ | ArsR/GlpF gel extraction | ||
+ | |||
+ | :: - Used zymo research Zymoclean TM Gel DNA Recovery Kit to extract ArsR, GlpF PCR2 and GlpF FullLenght from gel (cut from gel as shown in figure above). | ||
+ | |||
+ | '''GlpF PCR1''' | ||
+ | |||
+ | {| | ||
+ | PCR Mix | ||
+ | |12,5 uL | ||
+ | |Phusion | ||
+ | |- | ||
+ | |1 uL | ||
+ | |GlpF FW primer | ||
+ | |- | ||
+ | |1 uL | ||
+ | |GlpF RevMut primer | ||
+ | |- | ||
+ | |0,25 uL | ||
+ | |GlpF Full Lenght DNA (extracted as noted above)(not in tube2) | ||
+ | |- | ||
+ | |10,25 uL | ||
+ | |MQ | ||
+ | |} | ||
===Vectors=== | ===Vectors=== |
Revision as of 13:54, 27 July 2009
Wet
GVP Cluster
Transporters
Metal Accumulation
Gel with ArsR PCR((24/07/09)
ArsR/GlpF gel extraction
- - Used zymo research Zymoclean TM Gel DNA Recovery Kit to extract ArsR, GlpF PCR2 and GlpF FullLenght from gel (cut from gel as shown in figure above).
GlpF PCR1
12,5 uL | Phusion |
1 uL | GlpF FW primer |
1 uL | GlpF RevMut primer |
0,25 uL | GlpF Full Lenght DNA (extracted as noted above)(not in tube2) |
10,25 uL | MQ |
Vectors
Dry
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