Team:Groningen/Notebook/27 July 2009

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Revision as of 16:00, 27 July 2009 by JolandaWitteveen (Talk | contribs)

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Wet

GVP Cluster

Transporters

GlpF - PCR 2 was add on gel again as noted below at accumulation. GlpF PCR1

PCR1.1
Component amount
2x Phusion MM 12.5 uL
MQ 10.25 uL
GlpF Fw 1 uL
GlpF MutRev 1 uL
DNA GlpF Full Lenght (extracted as noted below at accumulation)(not added to tube nr2) 0.25 uL
GlpF PCR1 program Temperature Time
Denaturing 98° 2.00 min
Start Cycles 30X
Denaturing 98° 10 sec
Annealing 60° 10 sec
Elongation 72° 10 sec
End cycles
Final elongation 72° 5 min
Hold Forever


F102471 2009-07-27 16hr 21min GlpFPCR1 noted.JPG

Metal Accumulation

Gel with ArsR PCR((24/07/09)

270709 ArsR+GlpF.JPG

ArsR/GlpF gel extraction

- Used zymo research Zymoclean TM Gel DNA Recovery Kit to extract ArsR, GlpF PCR2 and GlpF FullLenght from gel (cut from gel as shown in figure above).

GlpF PCR1

PCR Mix
12,5 uL Phusion
1 uL GlpF FW primer
1 uL GlpF RevMut primer
0,25 uL GlpF Full Lenght DNA (extracted as noted above)(not in tube2)
10,25 uL MQ

Vectors

Dry

Finished RPU computations!


April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30