Team:Groningen/Notebook/28 August 2009

From 2009.igem.org

(Difference between revisions)
m (New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== :→ {{todo}} ===Transporters=== ===Metal Accumulation=== ===Vectors=== ==Dry== {{Team:Groningen/Notebook/Day/Foot...)
m (GVP Cluster)
Line 5: Line 5:
===GVP Cluster===
===GVP Cluster===
-
:→ {{todo}}  
+
:→ {{todo}} Isolate plasmids from o.n. cultures
 +
:→ {{todo}} Restriction analysis of plasmids for correct ligation of GVP behind LAC/pBAD promoter
 +
 
 +
:→ {{todo}} Test control of bouyancy in Saline solution
 +
:→ {{todo}} Order synthetic DNA for GVP
 +
 
 +
 
 +
'''Overnight Precultures'''
 +
 
 +
All tubes showed growth of E.coli cells, and must have ampicillin resistance on a plasmid(The tubes differed in cell density though). To be sure of correct ligation products in the plasmids an isolation will be performed, followed by restriction analysis. If positive, the plasmids will be sent for sequencing.
 +
 
 +
'''Overnight Plates'''
===Transporters===
===Transporters===

Revision as of 08:11, 28 August 2009

Igemhomelogo.png

Wet

GVP Cluster

TODO Isolate plasmids from o.n. cultures
TODO Restriction analysis of plasmids for correct ligation of GVP behind LAC/pBAD promoter
TODO Test control of bouyancy in Saline solution
TODO Order synthetic DNA for GVP


Overnight Precultures

All tubes showed growth of E.coli cells, and must have ampicillin resistance on a plasmid(The tubes differed in cell density though). To be sure of correct ligation products in the plasmids an isolation will be performed, followed by restriction analysis. If positive, the plasmids will be sent for sequencing.

Overnight Plates

Transporters

Metal Accumulation

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30