Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
m (Part List -80)
(Part List -80)
Line 40: Line 40:
|Ampicillin/Gentamycin
|Ampicillin/Gentamycin
|plate 3, pos.2
|plate 3, pos.2
 +
|-
 +
|(?)pHigh in low copy nr vector
 +
|High consitutive promoter
 +
|<partinfo>J23100</partinfo>
 +
|35bp
 +
|<partinfo>pSB3K3</partinfo>
 +
|Kanamycin
 +
|Plate 3, pos. 4
 +
|-
 +
|(?)pMed in low copy nr vector
 +
|Medium consitutive promoter
 +
|<partinfo>J23106</partinfo>
 +
|35bp
 +
|<partinfo>pSB3K3</partinfo>
 +
|Kanamycin
 +
|Plate 3, pos. 5
 +
|-
 +
|(?)pLow in low copy nr vector
 +
|Low consitutive promoter
 +
|<partinfo>J23109</partinfo>
 +
|35bp
 +
|<partinfo>pSB3K3</partinfo>
 +
|Kanamycin
 +
|Plate 3, pos. 6
 +
|-
 +
|(?)pHigh in high copy nr vector
 +
|High consitutive promoter
 +
|<partinfo>J23100</partinfo>
 +
|35bp
 +
|<partinfo>pSB1AC3</partinfo>
 +
|Kanamycin
 +
|Plate 3, pos. 7
 +
|-
 +
|(?)pMed in high copy nr vector
 +
|Medium consitutive promoter
 +
|<partinfo>J23106</partinfo>
 +
|35bp
 +
|<partinfo>pSB1AC3</partinfo>
 +
|Kanamycin
 +
|Plate 3, pos. 8
 +
|-
 +
|(?)pLow in high copy nr vector
 +
|High consitutive promoter
 +
|<partinfo>J23109</partinfo>
 +
|35bp
 +
|<partinfo>pSB1AC3</partinfo>
 +
|Kanamycin
 +
|Plate 3, pos. 9
|-
|-
|Promotor (low expression)
|Promotor (low expression)
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|Ampicillin
|Ampicillin
|plate 3, pos.12
|plate 3, pos.12
 +
|-
 +
|Inducible promoter
 +
|Lactose inducible promoter nr1
 +
|<partinfo>R0010</partinfo>
 +
|200bp
 +
|<partinfo>pSB1A2</partinfo>
 +
|Ampicillin
 +
|Plate 3, pos. 14
 +
|-
 +
|Inducible promoter
 +
|Lactose inducible promoter nr2
 +
|<partinfo>R0010</partinfo>
 +
|200bp
 +
|<partinfo>pSB1A2</partinfo>
 +
|Ampicillin
 +
|Plate 3, pos. 15
|-
|-
|RBS
|RBS

Revision as of 12:18, 25 July 2009

[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
Biobricks


Note

If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.

http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]


Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well
GVP Gas Vesicle Proteins [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] 6064 [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] Ampicillin/Gentamycin plate 3, pos.1
GVP (2nd batch) Gas Vesicle Proteins [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] 6064 [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] Ampicillin/Gentamycin plate 3, pos.2
(?)pHigh in low copy nr vector High consitutive promoter 35bp Kanamycin Plate 3, pos. 4
(?)pMed in low copy nr vector Medium consitutive promoter 35bp Kanamycin Plate 3, pos. 5
(?)pLow in low copy nr vector Low consitutive promoter 35bp Kanamycin Plate 3, pos. 6
(?)pHigh in high copy nr vector High consitutive promoter 35bp Kanamycin Plate 3, pos. 7
(?)pMed in high copy nr vector Medium consitutive promoter 35bp Kanamycin Plate 3, pos. 8
(?)pLow in high copy nr vector High consitutive promoter 35bp Kanamycin Plate 3, pos. 9
Promotor (low expression) Constitutive promotor [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] 35 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Ampicillin plate 3, pos.10
Promotor (medium expression) Constitutive promotor [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] 35 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Ampicillin plate 3, pos.11
Promotor (high expression) Constitutive promotor [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] 35 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Ampicillin plate 3, pos.12
Inducible promoter Lactose inducible promoter nr1 200bp Ampicillin Plate 3, pos. 14
Inducible promoter Lactose inducible promoter nr2 200bp Ampicillin Plate 3, pos. 15
RBS Ribosomal Binding Site [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] 12 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Ampicillin plate 3, pos.19
Terminator Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011] [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] ~90 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Ampicillin/Kanamycin plate 3, pos.20
HmtA (1.1) ? None ? None Ampicillin plate 3, pos.28
HmtA (2.1) ? None ? None Ampicillin plate 3, pos.29