Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
m
(Part List -80)
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|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.21
|plate 3, pos.21
-
|EcoRI/PstI + Sequenced
+
|EcoRI/PstI + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP (no.2)
|Const. Pormoter + GVP (no.2)
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|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.22
|plate 3, pos.22
-
|EcoRI/PstI + Sequenced
+
|EcoRI/PstI + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP
|Const. Pormoter + GVP
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|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.23
|plate 3, pos.23
-
|EcoRI/PstI + Sequenced
+
|EcoRI/PstI + {{done|Sequenced Okay}}
|-  
|-  
|Const. Pormoter + GVP (no.2)
|Const. Pormoter + GVP (no.2)
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|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
|plate 3, pos.24
|plate 3, pos.24
-
|EcoRI/PstI + Sequenced
+
|EcoRI/PstI + {{done|Sequenced Okay}}
|-  
|-  
|HmtA (1.1)
|HmtA (1.1)
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|Ampicillin
|Ampicillin
|plate 3, pos.37
|plate 3, pos.37
-
|Restriction(+) + Sequenced
+
|Restriction(+) + {{done|Sequenced Okay}}
|-  
|-  
|ZntR- + GVP in J61035
|ZntR- + GVP in J61035
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|Ampicillin
|Ampicillin
|plate 3, pos.38
|plate 3, pos.38
-
|Restriction(+) + Sequenced (3 mutations)
+
|Restriction(+) + {{done|Sequenced (3 mutations)}}
|-  
|-  
|ArsR- + GVP in J61035
|ArsR- + GVP in J61035
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|Ampicillin
|Ampicillin
|plate 3, pos.39
|plate 3, pos.39
-
|Restriction(+) + Sequenced
+
|Restriction(+) + {{done|Sequenced Okay}}
|-  
|-  
|ArsR- in J61002
|ArsR- in J61002
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|Ampicillin
|Ampicillin
|plate 3, pos.40
|plate 3, pos.40
-
|EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
+
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
|-  
|-  
|ZntR- in J61002
|ZntR- in J61002
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|Ampicillin
|Ampicillin
|plate 3, pos.41
|plate 3, pos.41
-
|EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
+
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced (3 mutations)}}
|-  
|-  
|CueO- in J61002
|CueO- in J61002
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|Ampicillin
|Ampicillin
|plate 3, pos.42
|plate 3, pos.42
-
|EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
+
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
|-  
|-  
|ZntR+RBS in J61002
|ZntR+RBS in J61002
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|Ampicillin
|Ampicillin
|plate 3, pos.43
|plate 3, pos.43
-
|EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
+
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
|-  
|-  
|ArsR+RBS in J61002
|ArsR+RBS in J61002
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|Ampicillin
|Ampicillin
|plate 3, pos.44
|plate 3, pos.44
-
|EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
+
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
|-  
|-  
|CueO+RBS in J61002
|CueO+RBS in J61002
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|Ampicillin
|Ampicillin
|plate 3, pos.45
|plate 3, pos.45
-
|EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
+
|EcoRI/SpeI(+), EcoRI/PstI(+), {{done|Sequenced Okay}}
|-
|-
|SmtA-GST in pGEX-3x
|SmtA-GST in pGEX-3x
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|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
|plate 3, pos. 59
|plate 3, pos. 59
-
|{{done|Sequence Okay}}
+
|{{done|Sequence 2 deletions between EcoRI and XbaI sites}}
|-
|-
|High consitutive promoter(with RFP)
|High consitutive promoter(with RFP)

Revision as of 14:15, 2 September 2009

[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
Biobricks


Note

If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.

http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]


Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] 6064 [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] 6064 [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter 35bp Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter 35bp Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter 35bp Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter 35bp Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] 35 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] 35 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] 35 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 200bp Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 200bp Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] 12 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011] [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] ~90 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Ampicillin/Kanamycin plate 3, pos.20 No
Const. Pormoter + GVP Low constitutive promoter with GVP cluster [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] ~6100 [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Low constitutive promoter with GVP cluster [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] ~6100 [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP Medium constitutive promoter with GVP cluster [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] ~6100 [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Medium constitutive promoter with GVP cluster [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] ~6100 [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI + Sequenced Okay
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+) + Sequenced Okay
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+) + Sequenced (3 mutations)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+) + Sequenced Okay
ArsR- in J61002 pArsR (promoter without RBS) BBa_K190015 71 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR- in J61002 pZntR (promoter without RBS) BBa_K190016 65 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
CueO- in J61002 pCueO (promoter without RBS) BBa_K190017 43 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR+RBS in J61002 pZntR (promoter with own RBS) BBa_K190022 91 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.43 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ArsR+RBS in J61002 pArsR (promoter with own RBS) BBa_K190023 81 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.44 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
CueO+RBS in J61002 pCueO (promoter with own RBS) BBa_K190024 71 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.45 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
SmtA-GST in pGEX-3x SmtA-GST fusion no biobrick primers available 1400bp pGEX-3x (NEB) Ampicillin plate 3, pos. 46 PCR, restriction
SmtA in pET29a SmtA 900bp pET29a (NEB) Kanamycin plate 3, pos. 47 PCR, restriction
MymT in pGB68 MymT 215bp Ampicillin pTXB1 (NEB) plate 3, pos. 48 PCR, restriction
GlpF and fMT in pMAL-MT GlpF and fMT on a synthetic operon in the vector and BBa_K1900?? (GlpF has no BioBrick nr yet) fMT = 200bp, GlpF = 900bp? Ampicillin pMALc2x (NEB) plate 3, pos. 49 PCR, restriction
MBP-ArsR MBP-ArsR Fusion protein 1500 bp pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 53 Restriction analysis] & PCR
pNL29 GVP Gene cluster 6000 bp pBluescriptIIKS Ampicillin plate 3, pos. 54 Buoyancy phenotype
pLac-fMT Metallothionein with inducible promotor Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 55 Quality control
pLow-fMT #4 Metallothionein with low constitutive promotor Part or Accession # Base Pairs (bp) pSB1A2 Ampicillin/chloramphenicol plate 3, pos. 56 Quality control
pLow-fMT #3 Metallothionein with low constitutive promotor Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 57 Quality control
SmtA Metallothionein Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 58 Quality control
fMT #6 Metallothionein for As3+ Part or Accession # Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 59 Sequence 2 deletions between EcoRI and XbaI sites
High consitutive promoter(with RFP) Constitutive promoter Bba_J23100 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 75 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Bba_J23106 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 76 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Bba_J23109 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 77 Quality control
High consitutive promoter(with RFP) Constitutive promoter Bba_J23100 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 78 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Bba_J23106 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 79 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Bba_J23109 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 80 Quality control
Reference constitutive promoter) Constitutive promoter ? Base Pairs (bp) ? ? plate 3, pos. 81 Quality control