Team:Groningen/Parts

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[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
Biobricks


Parts

TODOHere we need to give some genereal information about our parts.

  • For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen parts registry].
  • Here you can find an informative overview of our submitted parts.
  • Experience we had with parts from the registry can be found under used parts.


Note

If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.

http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]


Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins 6064 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins 6064 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter 35 Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter 35 Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter 35 Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter 35 Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter 35 Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter 35 Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor 35 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor 35 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor 35 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 200 Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 200 Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site 12 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of and ~90 Ampicillin/Kanamycin plate 3, pos.20 No
Const. Promoter + GVP Low constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI + Sequenced Okay
Const. Promoter + GVP (no.2) Low constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI + Sequenced Okay
Const. Promoter + GVP Medium constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI + Sequenced Okay
Const. Promoter + GVP (no.2) Medium constitutive promoter with GVP cluster + ~6100 Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI + Sequenced Okay
HmtA (1.1) HmtA-His in pBAD(a) None (to become ) 2004 [http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD] Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None (to become ) 2004 [http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD] Ampicillin plate 3, pos.29 Restriction(+)
RBS MBPArsR + None (to become ) ~1600 Ampicillin plate 3, pos.30,31,32 Restriction(+)PCR
pLac-fMT + Ars-RFP pArs testing (without the backbone) ~1400 Ampicillin/chloramphenicol plate 3, pos. 33,34,35 Quality control
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+) + Sequenced Okay
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+) + Sequenced (3 mutations)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+) + Sequenced Okay
ArsR- in J61002 pArsR (promoter without RBS) 71 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR- in J61002 pZntR (promoter without RBS) 65 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
CueO- in J61002 pCueO (promoter without RBS) 43 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR+RBS in J61002 pZntR (promoter with own RBS) 91 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.43 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ArsR+RBS in J61002 pArsR (promoter with own RBS) 81 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.44 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
CueO+RBS in J61002 pCueO (promoter with own RBS) 71 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.45 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
SmtA-GST in pGEX-3x SmtA-GST fusion no biobrick primers available 1400 [http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x] Ampicillin plate 3, pos. 46 PCR, restriction
SmtA in pET29a SmtA 900 [http://www.merckbiosciences.co.uk/product/69871 pET29a] Kanamycin plate 3, pos. 47 PCR, restriction
MymT in pGB68 MymT 215 [http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB) Ampicillin plate 3, pos. 48 PCR, restriction
GlpF and fMT in pMAL-MT GlpF and fMT on a synthetic operon in the vector and fMT = 200, GlpF = 900? [http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB) Ampicillin plate 3, pos. 49 PCR, restriction
MBP-ArsR MBP-ArsR Fusion protein 15180 Ampicillin/chloramphenicol plate 3, pos. 53 Restriction analysis & PCR
pNL29 GVP Gene cluster 6000 [http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS] Ampicillin plate 3, pos. 54 Buoyancy phenotype
pLac-fMT Metallothionein with inducible promotor 430 Ampicillin/chloramphenicol plate 3, pos. 55 Quality control
pLow-fMT #4 Metallothionein with low constitutive promotor 265 Ampicillin/chloramphenicol plate 3, pos. 56 Quality control
pLow-fMT #3 Metallothionein with low constitutive promotor 265 Ampicillin/chloramphenicol plate 3, pos. 57 Quality control
SmtA Metallothionein 678 Ampicillin/chloramphenicol plate 3, pos. 58 Quality control
fMT #6 Metallothionein for As3+ 222 Ampicillin/chloramphenicol plate 3, pos. 59 Sequence 2 deletions between EcoRI and XbaI sites
fMT #4 Metallothionein for As3+ 222 Ampicillin/chloramphenicol plate 3, pos. 60 Sequence Okay!
pZntR-GVP Metal promoter Base Pairs (bp) Kanamycin plate 3, pos. 69 Quality control
pCueO-GVP Metal promoter Base Pairs (bp) Kanamycin plate 3, pos. 70 Quality control
pLacI-GVP no.1 Inducible promoter Base Pairs (bp) Ampicillin plate 3, pos. 71 Quality control
pLacI-GVP no.2 Inducible promoter Base Pairs (bp) Ampicillin plate 3, pos. 72 Quality control
High consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 75 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 76 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Ampicillin/chloramphenicol plate 3, pos. 77 Quality control
High consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 78 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 79 Quality control
Low consitutive promoter(with RFP) Constitutive promoter Base Pairs (bp) Kanamycin plate 3, pos. 80 Quality control
Reference constitutive promoter) Constitutive promoter ? Base Pairs (bp) ? ? plate 3, pos. 81 Quality control