Team:HKU-HKBU/Parts

From 2009.igem.org

(Difference between revisions)
(Prototype team page)
(Parts submitted to the Registry)
 
(31 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{Team:HKU-HKBU/style.css}}
 +
{{Team:HKU-HKBU/script.js}}
 +
{{Team:HKU-HKBU/header}}
-
<html>
+
=Parts submitted to the Registry=
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
<!-- *** End of the alert box *** -->
+
'''This year, we constructed 50 and submitted to the Registry of Standard Parts a total of 42 new standard biobricks with code numbers from K283000 to K283049. Please click [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=HKU-HKBU Biobricks from HKU-HKBU 2009] for their detailed information. Of these biobricks, FORTY-FOUR contain complete information on primary nucleic acid sequence, description of function, authorship, safety notes and acknowedgment of sources and references; TWO (BBa_K283003 and BBa_K283005) are the improved versions of the existing biobricks, BBa_K094102 and BBa_J36846, rescpectively; THIRTY-THREE, including SEVEN devices, (see Registry for more information) were demonstrated work as expected in our system.'''
-
{|align="justify"
+
After constructing a serial of intermediates, such as BBa_K283003, BBa_K283006, BBa_K283030, and so on, we eventually generated ''four'' '''USEFUL and FUNCTIONAL''' devices (BBa_K283000, BBa_K283001, BBa_K283002, and BBa_K283014), in terms of polar expression system and speed controlling system. In polar expression system, BBa_K283000 and BBa_K283001 were designed to display eGFP-streptavidin fusion protein on the cell surface. BBa_K283000 contains a signal peptide of lipoprotein (''lpp'') and five transmembrane domains of outer membrane protein A (''ompA''), while BBa_K283001 have the AIDA transmembrane domain. '''Figure 1''' indicates the construction route of BBa_K283000. When expressed BBa_K283000 and BBa_K283001 in our ''Salmonalla'' strain YBS01 and ''E. coli'' strain YBE01, respectively, we clearly observed polar localization of eGFP-streptavidin protein ([https://2009.igem.org/Team:HKU-HKBU/Polar_Expression_Results#Polar_Expression see our polar expression results]).   
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
-
|[[Image:Example_logo.png|200px|right|frame]]
+
-
|-
+
-
|
+
-
''Tell us more about your project. Give us background.  Use this is the abstract of your projectBe descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:Team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:HKU-HKBU | Team Example]]
+
-
|}
+
-
<!--- The Mission, Experiments --->
 
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
[[Image:K283000new.jpg|center|thumb|700px|'''Figure 1.''' Assembly route of BBa_K283000]]
-
!align="center"|[[Team:HKU-HKBU|Home]]
+
-
!align="center"|[[Team:HKU-HKBU/Team|The Team]]
+
-
!align="center"|[[Team:HKU-HKBU/Project|The Project]]
+
-
!align="center"|[[Team:HKU-HKBU/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:HKU-HKBU/Modeling|Modeling]]
+
-
!align="center"|[[Team:HKU-HKBU/Notebook|Notebook]]
+
-
|}
+
-
(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
+
-
===Note===
 
-
If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
+
 +
To simultaneously control the swimming speed and propelling force of our engineered bacteria , we generated another two '''FUNCTIONAL''' devices BBa_K283002 and BBa_K283014. '''Figure 2''' shows the assembly route of BBa_K283002. When it was expressed in strain YBE01, a proportional relation was observed between the CheZ expression level/bacterial swimming speed and the concentration of inducer (IPTG in this case) ([https://2009.igem.org/Team:HKU-HKBU/Speed_Control_Results#Regulation_of_cheZ_expression see our speed control result]).
 +
 
 +
 
 +
[[Image:Assembly of BBa K283002new.jpg|center|thumb|700px|'''Figure 2.''' Assembly route of BBa_K283002]]
 +
 
 +
 
 +
 
 +
 
 +
{{Team:HKU-HKBU/footer}}

Latest revision as of 20:27, 21 October 2009

Parts submitted to the Registry

This year, we constructed 50 and submitted to the Registry of Standard Parts a total of 42 new standard biobricks with code numbers from K283000 to K283049. Please click [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=HKU-HKBU Biobricks from HKU-HKBU 2009] for their detailed information. Of these biobricks, FORTY-FOUR contain complete information on primary nucleic acid sequence, description of function, authorship, safety notes and acknowedgment of sources and references; TWO (BBa_K283003 and BBa_K283005) are the improved versions of the existing biobricks, BBa_K094102 and BBa_J36846, rescpectively; THIRTY-THREE, including SEVEN devices, (see Registry for more information) were demonstrated work as expected in our system.


After constructing a serial of intermediates, such as BBa_K283003, BBa_K283006, BBa_K283030, and so on, we eventually generated four USEFUL and FUNCTIONAL devices (BBa_K283000, BBa_K283001, BBa_K283002, and BBa_K283014), in terms of polar expression system and speed controlling system. In polar expression system, BBa_K283000 and BBa_K283001 were designed to display eGFP-streptavidin fusion protein on the cell surface. BBa_K283000 contains a signal peptide of lipoprotein (lpp) and five transmembrane domains of outer membrane protein A (ompA), while BBa_K283001 have the AIDA transmembrane domain. Figure 1 indicates the construction route of BBa_K283000. When expressed BBa_K283000 and BBa_K283001 in our Salmonalla strain YBS01 and E. coli strain YBE01, respectively, we clearly observed polar localization of eGFP-streptavidin protein (see our polar expression results).


Figure 1. Assembly route of BBa_K283000



To simultaneously control the swimming speed and propelling force of our engineered bacteria , we generated another two FUNCTIONAL devices BBa_K283002 and BBa_K283014. Figure 2 shows the assembly route of BBa_K283002. When it was expressed in strain YBE01, a proportional relation was observed between the CheZ expression level/bacterial swimming speed and the concentration of inducer (IPTG in this case) (see our speed control result).


Figure 2. Assembly route of BBa_K283002



Sponsors