Team:Heidelberg/k4l1um

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== 6-15-2009 ==
 
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Lab: LV, SH, CZ
 
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'''SH''':
 
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* [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)|Miniprep]] of GFP template plasmid, pcDNA5/FRT
 
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{| class="wikitable" border="1"
 
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|-
 
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!  Nr
 
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!  pcDNA5/FRT
 
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!  GFP
 
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|-
 
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|  1
 
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|  4,7
 
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|  39,7
 
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|-
 
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|  2
 
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|  5,9
 
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|  14,9
 
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|-
 
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|  3
 
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|  3,7
 
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|  3,8
 
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|-
 
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|  4
 
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|  5,0
 
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|  7,8
 
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|-
 
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|}
 
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* [[Team:Heidelberg/n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)|Maxiprep]] pcDNA/FRT 188.7ng/µL
 
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'''LV''':
 
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* Extraction of CMV promoter from 2008 distribution
 
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* [[Team:Heidelberg/n4tr1um# Transformation of Bacteria|Transformation]] of DH5a cell with CMV promoter
 
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* portzughtr
 
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== 6-16-2009 ==
 
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Lab: LV, SH, CZ
 
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* Annealing of Oligos (JeT, cFos, Min)
 
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* Purification of cDNA via 2% / 3% agarosegel
 
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* [[Team:Heidelberg/n4tr1um#Site-directed mutagenesis|Site directed mutagenesis]] of pcDNA5/FRT
 
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* Dpn1 digestion
 
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* Transformation of DH5a
 

Latest revision as of 12:24, 24 June 2009