Team:Heidelberg/k4l1um

From 2009.igem.org

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(6-15-2009)
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'''SH''':  
'''SH''':  
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* Miniprep of GFP template plasmid, pcDNA5/FRT
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* [[n4tr1um#Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)]]Miniprep of GFP template plasmid, pcDNA5/FRT
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Revision as of 13:36, 17 June 2009

6-15-2009

Lab: LV, SH, CZ

SH:

Nr pcDNA5/FRT GFP
1 4,7 39,7
2 5,9 14,9
3 3,7 3,8
4 5,0 7,8
  • Maxiprep pcDNA/FRT 188.7ng/µL


LV:

  • Extraction of CMV promoter from 2008 distribution
  • Transformation of DH5a cell with CMV promoter
  • portzughtr

6-16-2009

Lab: LV, SH, CZ

  • Annealing of oligos (JeT, cFos, Min)
  • Purification of cDNA via 2% / 3% agarosegel
  • Site directed mutagenesis of pcDNA5/FRT
  • Dpn1 digestion
  • Transformation of DH5a