Team:IBB Pune/project/systems together

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Latest revision as of 02:26, 22 October 2009

Master Plan

Constructing multi-strain computational modules using Nucleotide and Protein mediated cell-cell signaling

Building complex genetic circuits in a single cell becomes difficult due to the formidable task of co-transforming large nucleotide sequences in addition to the imposed metabolic burden on the cell. Can a complex system be divided into independent modules that reside in different cells and interact with each other using nucleotide and protein mediated cell-cell signalling to act as a single unit? We seek to address this problem using a three pronged approach.

Firstly, we are trying to introduce natural competance genes into the biobrick framework which will act as nucleotide importers.

Secondly, we are building a protein export system using the TAT dependent export pathway.

Finally, we are attempting to construct a multi-state turing machine which is a compound, modular computational system that has independent, interacting states which applies the above principle. We hope that this approach overcomes the obstacles in building more complex and composite circuits.

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+<html><p><span style="font-weight:bold; font-size:200%; color:#0000cc;">Master Plan</span></p></html>
+<html><p><span style="font-weight:bold; font-size:125%; color:#FF6600;">Constructing multi-strain computational modules using Nucleotide and Protein mediated cell-cell signaling</span></p></html>
+Building complex genetic circuits in a single cell becomes difficult due to the formidable task of co-transforming large nucleotide sequences in addition to the imposed metabolic burden on the cell. Can a complex system be divided into independent modules that reside in different cells and interact with each other using nucleotide and protein mediated cell-cell signalling to act as a single unit? We seek to address this problem using a three pronged approach.
+Firstly, we are trying to introduce natural competance genes into the biobrick framework which will act as nucleotide importers.
+Secondly, we are building a protein export system using the TAT dependent export pathway.
+Finally, we are attempting to construct a multi-state turing machine which is a compound, modular computational system that has independent, interacting states which applies the above principle. We hope that this approach overcomes the obstacles in building more complex and composite circuits.