Team:IBB Pune/protein-based-signalling

From 2009.igem.org

(Difference between revisions)
(Working of the Construct)
(Working of the Construct)
Line 10: Line 10:
* A constitutively working module which produces LuxR protein and LacI
* A constitutively working module which produces LuxR protein and LacI
*A State-'B'  
*A State-'B'  
-
*A
+
*An AND gate regulated activation of the turing machine function (it could be lysis, expression of a fluorescent protein or the synthesis of vanillin or methyl salicylate)
 +
 
 +
The default state of the turing machine  is 'A'.
 +
In this state it encounters a '0' (represented by Lactose) first.
=Problems Associated with such an Approach=
=Problems Associated with such an Approach=

Revision as of 21:55, 21 October 2009

Contents

The Complete Turing Machine

BBa K233325.JPG

The above image is a representation of the complete turing machine including inter state regulation. This model is true to Alan Turing's vision of multi-mutually exclusive states and the states being regulated by one another through outputs.

Working of the Construct

In the above construct, there are three independent modules that function

  • A constitutively working module which produces LuxR protein and LacI
  • A State-'B'
  • An AND gate regulated activation of the turing machine function (it could be lysis, expression of a fluorescent protein or the synthesis of vanillin or methyl salicylate)

The default state of the turing machine is 'A'. In this state it encounters a '0' (represented by Lactose) first.

Problems Associated with such an Approach

Modular Systems

Proof-of-concept

Relation to Project 1