Team:IBB Pune/protein-based-signalling

From 2009.igem.org

(Difference between revisions)
(Working of the Construct)
(Working of the Construct)
Line 13: Line 13:
The default state of the turing machine  is 'A'.
The default state of the turing machine  is 'A'.
-
In this state it encounters a '0' (represented by Lactose) first.
+
In this state it encounters a '0' (represented by Lactose) first.However there are no Lactose responsive elements in either the first or the second module.
 +
The third Module which has such a module however also requires the presence of
=Problems Associated with such an Approach=
=Problems Associated with such an Approach=

Revision as of 21:56, 21 October 2009

Contents

The Complete Turing Machine

BBa K233325.JPG

The above image is a representation of the complete turing machine including inter state regulation. This model is true to Alan Turing's vision of multi-mutually exclusive states and the states being regulated by one another through outputs.

Working of the Construct

In the above construct, there are three independent modules that function

  • A constitutively working module which produces LuxR protein and LacI
  • A State-'B'
  • An AND gate regulated activation of the turing machine function (it could be lysis, expression of a fluorescent protein or the synthesis of vanillin or methyl salicylate)

The default state of the turing machine is 'A'. In this state it encounters a '0' (represented by Lactose) first.However there are no Lactose responsive elements in either the first or the second module. The third Module which has such a module however also requires the presence of

Problems Associated with such an Approach

Modular Systems

Proof-of-concept

Relation to Project 1