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Team:Imperial College London/Notebook
From 2009.igem.org
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{{Imperial/09/TemplateTop}} | {{Imperial/09/TemplateTop}} | ||
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=Notebook= | =Notebook= | ||
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Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments. <br> | Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments. <br> | ||
<br> | <br> | ||
| - | {{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09}} | + | {{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09 | left}}{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=10 | left}} |
{{Imperial/09/Division}} | {{Imperial/09/Division}} | ||
| - | =Progress= | + | |
| + | =BioBrick Progress= | ||
The table below shows the progress/status of each BioBrick part/construct: | The table below shows the progress/status of each BioBrick part/construct: | ||
| Line 15: | Line 17: | ||
{| width="100%" style="background-color:transparent;" | {| width="100%" style="background-color:transparent;" | ||
| | | | ||
| - | {| border="2" cellpadding="2" style="text-align: | + | {| border="2" cellpadding="2" style="text-align:left" align="left" width="30%" |
! Key | ! Key | ||
|- style=background:LimeGreen | |- style=background:LimeGreen | ||
| Line 22: | Line 24: | ||
| Sequenced | | Sequenced | ||
|- style=background:MediumSlateBlue | |- style=background:MediumSlateBlue | ||
| - | | Midi- | + | | Midi-prepped |
|- style=background:GreenYellow | |- style=background:GreenYellow | ||
| Fully verified | | Fully verified | ||
|- style=background:MediumVioletRed | |- style=background:MediumVioletRed | ||
| - | | Partially | + | | Partially verified |
| - | |- style=background: | + | |- style=background:Orchid |
| Transformed | | Transformed | ||
|- style=background:DarkOrange | |- style=background:DarkOrange | ||
| Line 35: | Line 37: | ||
|- style=background:Red | |- style=background:Red | ||
| Site-directed mutagenesis | | Site-directed mutagenesis | ||
| - | |- style=background: | + | |- style=background:YellowGreen |
| Awaiting Mr.Gene | | Awaiting Mr.Gene | ||
|} | |} | ||
| Line 42: | Line 44: | ||
{| border="2" cellpadding="2" style="text-align:left" align="left" width="100%" | {| border="2" cellpadding="2" style="text-align:left" align="left" width="100%" | ||
!Part Number !! Part Name !! Status | !Part Number !! Part Name !! Status | ||
| - | |- style=background: | + | |- style=background:Red |
| - | | BBa_K200000 ||RcsB|| Sequenced | + | | BBa_K200000 ||RcsB|| Sequenced from mini-prep/SDM on midi-prep |
|- style=background:MediumVioletRed | |- style=background:MediumVioletRed | ||
| - | | BBa_K200001 ||Dam methylase|| Mini- | + | | BBa_K200001 ||Dam methylase|| Mini-prepped |
|- style=background:MediumVioletRed | |- style=background:MediumVioletRed | ||
| - | | BBa_K200002 ||ygiV (B3023)|| Mini- | + | | BBa_K200002 ||ygiV (B3023)|| Mini-prepped |
|- style=background:MediumVioletRed | |- style=background:MediumVioletRed | ||
| - | | BBa_K200003 ||Waal Ligase|| Mini- | + | | BBa_K200003 ||Waal Ligase|| Mini-prepped |
|- style=background:MediumSlateBlue | |- style=background:MediumSlateBlue | ||
| - | | BBa_K200005 ||OtsA|| Sequenced | + | | BBa_K200005 ||OtsA|| Sequenced from midi-prep |
|- style=background:MediumSlateBlue | |- style=background:MediumSlateBlue | ||
| - | | BBa_K200006 ||OtsB|| Sequenced | + | | BBa_K200006 ||OtsB|| Sequenced from midi-prep |
| - | |- style=background: | + | |- style=background:Orchid |
| BBa_K200007 ||Cellulase|| Transformed | | BBa_K200007 ||Cellulase|| Transformed | ||
|- style=background:Red | |- style=background:Red | ||
| - | | BBa_K200008 ||Phenylalanine | + | | BBa_K200008 ||Phenylalanine hydroxylase|| Site-directed mutagenesis/mini-preps |
| - | |- style=background: | + | |- style=background:YellowGreen |
| - | | BBa_K200009 ||DpnII | + | | BBa_K200009 ||DpnII restriction enzyme|| Awaiting Mr. Gene |
| - | |- style=background: | + | |- style=background:Orchid |
| - | | BBa_K200009 ||TaqI | + | | BBa_K200009 ||TaqI restriction enzyme (RBS+TaqI+TT)|| Transformed |
| - | |- style=background: | + | |- style=background:YellowGreen |
| BBa_K200011 ||Lambda cI repressor|| Awaiting Mr. Gene | | BBa_K200011 ||Lambda cI repressor|| Awaiting Mr. Gene | ||
|- style=background:DeepSkyBlue | |- style=background:DeepSkyBlue | ||
| - | | BBa_K200012 ||Lambda promoter|| Sequenced | + | | BBa_K200012 ||Lambda promoter|| Sequenced from mini-prep |
|- style=background:DeepSkyBlue | |- style=background:DeepSkyBlue | ||
| - | | BBa_ ||pLacI|| Sequenced | + | | BBa_ ||pLacI|| Sequenced from mini-prep |
|- style=background:DeepSkyBlue | |- style=background:DeepSkyBlue | ||
| - | | BBa_ ||pCstA|| Sequenced | + | | BBa_ ||pCstA|| Sequenced from mini-prep |
| - | |- style=background: | + | |- style=background:Orchid |
| - | | BBa_ ||pCstA+RBS+ | + | | BBa_ ||pCstA+RBS+GFP+TT|| Transformed |
| + | |- style=background:Orchid | ||
| + | | BBa_ ||pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT|| Transformed | ||
| + | |- style=background:DarkOrange | ||
| + | | BBa_ ||RBS+OtsB|| Mini-prepped | ||
| + | |- style=background:MediumVioletRed | ||
| + | | BBa_ ||pCstA+RBS|| Mini-prepped | ||
| + | |- style=background:MediumVioletRed | ||
| + | | BBa_ ||LacI+RBS|| Mini-prepped | ||
|} | |} | ||
Latest revision as of 21:02, 13 October 2009
- Back to Hub
- Cloning Strategy
- Protocols
- BioBricks
- Results
- Notebook
Notebook
Here is our daily notebook.
Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments.
| September | ||||||
| M | T | W | T | F | S | S |
| [http://2009.igem.org/Imperial_College_London/Notebook/1_September_2009 1] | [http://2009.igem.org/Imperial_College_London/Notebook/2_September_2009 2] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/3_September_2009&action=edit 3] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/4_September_2009&action=edit 4] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/5_September_2009&action=edit 5] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/6_September_2009&action=edit 6] | |
| [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/7_September_2009&action=edit 7] | [http://2009.igem.org/Imperial_College_London/Notebook/8_September_2009 8] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/9_September_2009&action=edit 9] | [http://2009.igem.org/Imperial_College_London/Notebook/10_September_2009 10] | [http://2009.igem.org/Imperial_College_London/Notebook/11_September_2009 11] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/12_September_2009&action=edit 12] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/13_September_2009&action=edit 13] |
| [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/14_September_2009&action=edit 14] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/15_September_2009&action=edit 15] | [http://2009.igem.org/Imperial_College_London/Notebook/16_September_2009 16] | [http://2009.igem.org/Imperial_College_London/Notebook/17_September_2009 17] | [http://2009.igem.org/Imperial_College_London/Notebook/18_September_2009 18] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/19_September_2009&action=edit 19] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/20_September_2009&action=edit 20] |
| [http://2009.igem.org/Imperial_College_London/Notebook/21_September_2009 21] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/22_September_2009&action=edit 22] | [http://2009.igem.org/Imperial_College_London/Notebook/23_September_2009 23] | [http://2009.igem.org/Imperial_College_London/Notebook/24_September_2009 24] | [http://2009.igem.org/Imperial_College_London/Notebook/25_September_2009 25] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/26_September_2009&action=edit 26] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/27_September_2009&action=edit 27] |
| [http://2009.igem.org/Imperial_College_London/Notebook/28_September_2009 28] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/29_September_2009&action=edit 29] | [http://2009.igem.org/Imperial_College_London/Notebook/30_September_2009 30] | ||||
| October | ||||||
| M | T | W | T | F | S | S |
| [http://2009.igem.org/Imperial_College_London/Notebook/1_October_2009 1] | [http://2009.igem.org/Imperial_College_London/Notebook/2_October_2009 2] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/3_October_2009&action=edit 3] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/4_October_2009&action=edit 4] | |||
| [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/5_October_2009&action=edit 5] | [http://2009.igem.org/Imperial_College_London/Notebook/6_October_2009 6] | [http://2009.igem.org/Imperial_College_London/Notebook/7_October_2009 7] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/8_October_2009&action=edit 8] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/9_October_2009&action=edit 9] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/10_October_2009&action=edit 10] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/11_October_2009&action=edit 11] |
| [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/12_October_2009&action=edit 12] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/13_October_2009&action=edit 13] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/14_October_2009&action=edit 14] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/15_October_2009&action=edit 15] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/16_October_2009&action=edit 16] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/17_October_2009&action=edit 17] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/18_October_2009&action=edit 18] |
| [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/19_October_2009&action=edit 19] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/20_October_2009&action=edit 20] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/21_October_2009&action=edit 21] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/22_October_2009&action=edit 22] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/23_October_2009&action=edit 23] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/24_October_2009&action=edit 24] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/25_October_2009&action=edit 25] |
| [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/26_October_2009&action=edit 26] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/27_October_2009&action=edit 27] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/28_October_2009&action=edit 28] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/29_October_2009&action=edit 29] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/30_October_2009&action=edit 30] | [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/31_October_2009&action=edit 31] | |
BioBrick Progress
The table below shows the progress/status of each BioBrick part/construct:
|
| Part Number | Part Name | Status |
|---|---|---|
| BBa_K200000 | RcsB | Sequenced from mini-prep/SDM on midi-prep |
| BBa_K200001 | Dam methylase | Mini-prepped |
| BBa_K200002 | ygiV (B3023) | Mini-prepped |
| BBa_K200003 | Waal Ligase | Mini-prepped |
| BBa_K200005 | OtsA | Sequenced from midi-prep |
| BBa_K200006 | OtsB | Sequenced from midi-prep |
| BBa_K200007 | Cellulase | Transformed |
| BBa_K200008 | Phenylalanine hydroxylase | Site-directed mutagenesis/mini-preps |
| BBa_K200009 | DpnII restriction enzyme | Awaiting Mr. Gene |
| BBa_K200009 | TaqI restriction enzyme (RBS+TaqI+TT) | Transformed |
| BBa_K200011 | Lambda cI repressor | Awaiting Mr. Gene |
| BBa_K200012 | Lambda promoter | Sequenced from mini-prep |
| BBa_ | pLacI | Sequenced from mini-prep |
| BBa_ | pCstA | Sequenced from mini-prep |
| BBa_ | pCstA+RBS+GFP+TT | Transformed |
| BBa_ | pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT | Transformed |
| BBa_ | RBS+OtsB | Mini-prepped |
| BBa_ | pCstA+RBS | Mini-prepped |
| BBa_ | LacI+RBS | Mini-prepped |
