Team:KU Seoul/Parts

From 2009.igem.org

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=== Part ===
=== Part ===
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::'''Mercury sensor - Mercury inducing promoter''' {{Part|BBa_K271000}} = Part:{{Part|BBa_I728456}} + amd gene  
+
::'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}})
[[Image:amd_part.png]]
[[Image:amd_part.png]]
:Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations,  
:Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations,  

Revision as of 14:07, 21 October 2009






Part

Mercury sensor - Mercury inducing promoter Part:[http://partsregistry.org/Part:BBa_I728456 BBa_I728456] + amd gene([http://partsregistry.org/Part:BBa_K271000 BBa_K271000])

Amd part.png

Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations,
cells are able to adapt and resume growth after a period of stasis. Promoter yodA is only transiently activated
during cadmium-induced growth inhibition. Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the
amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing
a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having
amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-
purple color, aryl acylamidase gene will apply to reporter gene.
Heavy-metal detector [http://partsregistry.org/Part:BBa_K271001 BBa_K271001]
The promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer
of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the
promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat
sequence.
Zinc part.png