Team:KU Seoul/Parts

From 2009.igem.org

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::'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}})  
::'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}})  
[[Image:amd_part.png]]
[[Image:amd_part.png]]
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:Cadmium is an exceedingly toxic heavy metal with no known biological function. At low cadmium concentrations,  
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:Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.  
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:cells are able to adapt and resume growth after a period of stasis. Promoter yodA is only transiently activated  
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:during cadmium-induced growth inhibition. Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the  
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Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.  
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:amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing  
+
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:a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having  
+
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:amide bond is hydrolyzed by the enzyme and produce acetate and p-aminophenol. Because p-Aminophenol have red-
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:purple color, aryl acylamidase gene will apply to reporter gene.  
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:'''Heavy-metal detector {{Part|BBa_K271001}}'''
:'''Heavy-metal detector {{Part|BBa_K271001}}'''
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:The promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer  
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:The promoter/operator region of the zntA gene of Escherichia coli. It shows that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat sequence.  
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:of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the  
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:promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat  
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:sequence.  
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::[[Image:zinc_part.png]]
::[[Image:zinc_part.png]]

Revision as of 15:50, 21 October 2009






Part

Mercury sensor - Mercury inducing promoter Part:[http://partsregistry.org/Part:BBa_I728456 BBa_I728456] + amd gene([http://partsregistry.org/Part:BBa_K271000 BBa_K271000])

Amd part.png

Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.

Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.

Heavy-metal detector [http://partsregistry.org/Part:BBa_K271001 BBa_K271001]
The promoter/operator region of the zntA gene of Escherichia coli. It shows that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat sequence.
Zinc part.png