Team:Kyoto/CiC/Notebook/0921-0925

From 2009.igem.org

(Difference between revisions)
(New page: {{:Team:Kyoto/Header2}} <html> <script type="text/javascript"> <!-- $(function(){ initialize("CiC"); // <== initialize("GSDD" or "CiC"); 1) }); // --> </script> <style type="text/c...)
(Monday, 14 August)
Line 31: Line 31:
==0914-0918 : what to do. what to do. what to do. what to do. ==
==0914-0918 : what to do. what to do. what to do. what to do. ==
{{:Team:Kyoto/Pad_start_dates}}
{{:Team:Kyoto/Pad_start_dates}}
-
===Monday, 14 August===
+
===Monday, 21 September===
====To Do====
====To Do====
-
*p-2-(1)(4)(7)(8)(10)
+
*HIV-TAT-(LALAAAA)3-generator
-
**Miniprep
+
-
**Restriction Enzyme Digestion
+
-
*p-2-(1)(4)  E.X
+
-
**PCR purification kit
+
-
*p-2-(1)(7)(8)(10)  E.S
+
-
**Gel extraction
+
-
*4-(1)(2)(3)
+
**Colony PCR
**Colony PCR
-
**Insert check
 
-
 
====Results====
====Results====
-
=====p-2-(1)(4)(7)(8)(10)=====
+
*消えた
-
=====p-2-(1)(7)(8)(10)  E.S=====
+
{{:Team:Kyoto/Pad_between_dates}}
-
*Gel extraction kit
+
-
[[Image:Kyoto_0914_PCR.png|250px]]
+
-
{| class="table"
+
-
|+Miniprep product conc.
+
-
!NO.!!Sample name!!conc./(ng/ul)
+
-
|-
+
-
|1||p-2-(1)||9.3
+
-
|-
+
-
|2||p-2-(7)||25.9
+
-
|-
+
-
|3||p-2-(8)||8.6
+
-
|-
+
-
|4||p-2-(10)||7.5
+
-
|}
+
-
=====4-(1)(2)(3)=====
 
-
[[Image:Kyoto_0914_PCR_2.png|250px]]
 
-
*This result indicates that (1)a, (2)b and (3)a were inserted properly.
 
-
 
-
{{:Team:Kyoto/Pad_between_dates}}
 
===Tuesday, 15 September===
===Tuesday, 15 September===
====To Do====
====To Do====

Revision as of 11:36, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.


Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • 消えた



Tuesday, 15 September

To Do

Further to 14 Sept.

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check



Wednesday, 16 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)