Team:Kyoto/CiC/Notebook/0921-0925

From 2009.igem.org

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(→To Do)
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===Wednesday, 23 September===
===Wednesday, 23 September===
====To Do====
====To Do====
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*sig-EGFP generator
**ligation
**ligation
**transformation
**transformation
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!NO.!!Sample name!!conc./(ng/ul)
!NO.!!Sample name!!conc./(ng/ul)
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|-
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|1||11(stu1)||103.2
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|1||pEGFP-N3(EcoR1,Xho1)(||224.3
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|2||signal sequence(||38.1
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Revision as of 17:03, 21 October 2009

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  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.

Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • Binds of expected size were observed.

Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • sig-EGFP generator(HeLa)
    • PEGFP-N3 VECTOR (EGFP generator)

Results

  • 消えた

Wednesday, 23 September

To Do

  • sig-EGFP generator
    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
1pEGFP-N3(EcoR1,Xho1)(224.3
2signal sequence(38.1
Transformation

No colony was observed (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0921-0925"