Team:Kyoto/CiC/Notebook/0921-0925

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==0914-0918 : what to do. what to do. what to do. what to do. ==
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==0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)  ==
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===Monday, 21 September===
===Monday, 21 September===
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=====Transformation=====
=====Transformation=====
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No colony was observed (090917 10:00) 
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No colony was observed. (090917 10:00) 
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Latest revision as of 02:06, 22 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)


Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • Binds of expected size were observed.



Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • sig-EGFP generator(HeLa)
    • Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
    • hosphorylation signal sequence primer
    • annering signal sequence primer



Wednesday, 23 September

To Do

  • sig-EGFP generator
    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)conc.(ul)
1pEGFP-N3(EcoR1,Xho1)224.31
2signal sequence0.311
Transformation

No colony was observed. (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)