Team:Kyoto/CiC/Notebook/1005-1009

From 2009.igem.org

(Difference between revisions)
(Transformation)
(Thursday, 08 October)
Line 60: Line 60:
===Thursday, 08 October===
===Thursday, 08 October===
====To Do====
====To Do====
 +
*sig-GFP generator
 +
**Restriction Enzyme
 +
**ligation
 +
**transformation
 +
*sig-EGFP generator
 +
**phosphorylation signal sequence primer
 +
**anneling signal sequence primer
 +
**ligation
 +
**transformation
====Results====
====Results====
 +
=====Transformation=====
 +
Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}

Revision as of 15:32, 21 October 2009

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  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1005-1009 : what to do. what to do. what to do. what to do.


Monday, 05 October

To Do

No experiments were undertaken



Tuesday, 06 October

To Do

No experiments were undertaken



Wednesday, 07 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)



Thursday, 08 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)



Friday, 09 October

To Do

Results



Saturday, 10 October

To Do

Results



Saturday, 11 October

To Do

Results



Sunday, 12 October

To Do

Results