Team:Kyoto/CiC/Notebook/1005-1009

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-
==0914-0918 : what to do. what to do. what to do. what to do. ==
+
==1005-1009 : constructing sig-GFP generator,sig-EGFP generator(HeLa)  ==
 +
 
{{:Team:Kyoto/Pad_start_dates}}
{{:Team:Kyoto/Pad_start_dates}}
-
===Monday, 14 August===
+
===Monday, 05 October===
====To Do====
====To Do====
-
*p-2-(1)(4)(7)(8)(10)
+
No experiments were undertaken
-
**Miniprep
+
-
**Restriction Enzyme Digestion
+
-
*p-2-(1)(4)  E.X
+
-
**PCR purification kit
+
-
*p-2-(1)(7)(8)(10)  E.S
+
-
**Gel extraction
+
-
*4-(1)(2)(3)
+
-
**Colony PCR
+
-
**Insert check
+
-
 
+
-
====Results====
+
-
=====p-2-(1)(4)(7)(8)(10)=====
+
-
=====p-2-(1)(7)(8)(10)  E.S=====
+
-
*Gel extraction kit
+
-
[[Image:Kyoto_0914_PCR.png|250px]]
+
-
{| class="table"
+
-
|+Miniprep product conc.
+
-
!NO.!!Sample name!!conc./(ng/ul)
+
-
|-
+
-
|1||p-2-(1)||9.3
+
-
|-
+
-
|2||p-2-(7)||25.9
+
-
|-
+
-
|3||p-2-(8)||8.6
+
-
|-
+
-
|4||p-2-(10)||7.5
+
-
|}
+
-
 
+
-
=====4-(1)(2)(3)=====
+
-
[[Image:Kyoto_0914_PCR_2.png|250px]]
+
-
*This result indicates that (1)a, (2)b and (3)a were inserted properly.
+
-
 
+
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
-
===Tuesday, 15 September===
 
-
====To Do====
 
-
'''Further to 14 Sept.'''
 
-
 
-
*p-2-(1)(4)(7)(8)(10)
 
-
**Miniprep
 
-
**Restriction Enzyme Digestion
 
-
*p-2-(1)(4)  E.X
 
-
**PCR purification kit
 
-
*p-2-(1)(7)(8)(10)  E.S
 
-
**Gel extraction
 
-
*4-(1)(2)(3)
 
-
**Colony PCR
 
-
**Insert check
 
 +
===Tuesday, 06 October===
 +
====To Do====
 +
No experiments were undertaken
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
-
===Wednesday, 16 September===
+
 
 +
===Wednesday, 07 October===
====To Do====
====To Do====
 +
*sig-GFP generator
 +
**Restriction Enzyme
 +
**ligation
 +
**transformation
 +
*sig-EGFP generator
 +
**phosphorylation signal sequence primer
 +
**anneling signal sequence primer
**ligation
**ligation
**transformation
**transformation
-
 
====Results====
====Results====
-
=====ligation=====
+
=====Transformation=====
-
{| class="table"
+
Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)
-
|+conc.
+
{{:Team:Kyoto/Pad_between_dates}}
-
!NO.!!Sample name!!conc./(ng/ul)
+
-
|-
+
-
|1||11(stu1)||103.2
+
-
|}
+
 +
===Thursday, 08 October===
 +
====To Do====
 +
*sig-GFP generator
 +
**Restriction Enzyme
 +
**ligation
 +
**transformation
 +
*sig-EGFP generator
 +
**phosphorylation signal sequence primer
 +
**anneling signal sequence primer
 +
**ligation
 +
**transformation
 +
====Results====
=====Transformation=====
=====Transformation=====
-
No colony was observed (090917 10:00)　
+
Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010)
 +
{{:Team:Kyoto/Pad_between_dates}}
 +
===Friday, 09 October===
 +
====To Do====
 +
*No experiments were undertaken
 +
{{:Team:Kyoto/Pad_between_dates}}
 +
 +
===Saturday, 10 October===
 +
====To Do====
 +
*sig-GFP
 +
**miniprep
 +
**Restriction Enzyme
 +
*sig-EGFP(HeLa)
 +
**miniprep
 +
**Restriction Enzyme
 +
====Results====
 +
Plasmid construction is completed!!
 +
{{:Team:Kyoto/Pad_between_dates}}
 +
 +
===Saturday, 11 October===
 +
====To Do====
 +
*Yeast Mitochondria isolation
 +
====Results====
 +
*It is too difficult for us to isolate mitochondoria.
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
-
===Thursday, 17 September===
+
===Sunday, 12 October===
====To Do====
====To Do====
-
*Make repetitive sequence (MPR)
+
*No experiments were undertaken
-
*Transformation(retry)
+
{{:Team:Kyoto/Pad_endof_dates}}
{{:Team:Kyoto/Pad_endof_dates}}
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Latest revision as of 19:31, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1005-1009 : constructing sig-GFP generator,sig-EGFP generator(HeLa)

Monday, 05 October

To Do

No experiments were undertaken

Tuesday, 06 October

To Do

No experiments were undertaken

Wednesday, 07 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)

Thursday, 08 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010)

Friday, 09 October

To Do

  • No experiments were undertaken

Saturday, 10 October

To Do

  • sig-GFP
    • miniprep
    • Restriction Enzyme
  • sig-EGFP(HeLa)
    • miniprep
    • Restriction Enzyme

Results

Plasmid construction is completed!!

Saturday, 11 October

To Do

  • Yeast Mitochondria isolation

Results

  • It is too difficult for us to isolate mitochondoria.

Sunday, 12 October

To Do

  • No experiments were undertaken

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/1005-1009"