Wet Lab!!

General objectives

I. Biobrick Assembly of the Kamikaze system

Many cuts, pastes and clones for biobrick organization into the suicide system!!

II. Construction the standardized P4 vector

An incredible and challenging fight against PCR and logical thinking!!

III. Construction of the P4-producing strain

A passionate struggle with natural selection and recombination!!

IV. System testing and parameter obtention

The integrative side of the bite back

Personal Objectives

Uriel Urquiza

-Construction of the phage production control system.
 
  In order to produce a grate amount of P4 phage particles that has our death system, we want to avoid the natural
  early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the two
  major regulators of P2 morphopoietic genes. The control systems that is going to be implemented is constituted by a 
  promoter inducible by IPTG () in conjunction with transactivators cox and ogr from phage P2. All this will allow 
  us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence obtation of grate
  amounts of P4 phage.

Work Journals

• Nando's Lab Journal

• Uriel's Lab Journal

• Abraham's Lab Journal

• Laura's Lab Journal

• Paz Paz