Team:LCG-UNAM-Mexico/Wet Lab/Objectives
From 2009.igem.org
(Difference between revisions)
(→Abraham Avelar) |
(→Fernando Montaño) |
||
Line 80: | Line 80: | ||
====Fernando Montaño==== | ====Fernando Montaño==== | ||
<br> | <br> | ||
- | + | ====Assembly of the P4 vector.====<br> | |
* I must create an eternal bacterial source of P4 sid1. Therefore I must Infect C1a with it. | * I must create an eternal bacterial source of P4 sid1. Therefore I must Infect C1a with it. | ||
*I need to develop a good protocol for P4 sid1 lysate production. | *I need to develop a good protocol for P4 sid1 lysate production. | ||
Line 87: | Line 87: | ||
4) When the kamikaze system is ready, I need to ligate the P4sid1 region to it and transform an E.coli C1a strain with it. <br> | 4) When the kamikaze system is ready, I need to ligate the P4sid1 region to it and transform an E.coli C1a strain with it. <br> | ||
5) then we can start testing the system!<br> | 5) then we can start testing the system!<br> | ||
- | 6) ultimate goal: standardize P4 as an iGEM vector | + | 6) ultimate goal: standardize P4 as an iGEM vector. |
+ | |||
+ | ====Testing for activity of the control system==== | ||
+ | |||
+ | Uriel's cox and ogr construction needs to be tested experimentally to assureThe procedure will be: | ||
+ | |||
+ | 1) Transform a P2 lysogenic strain with the construction. | ||
+ | 2)assess IPTG induced lysis activation | ||
====Enrique Paz==== | ====Enrique Paz==== |
Revision as of 21:41, 21 October 2009