Team:LCG-UNAM-Mexico/Wet Lab/Objectives
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(→Abraham Avelar) |
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In order to produce a grate amount of P4 phage particles that has our death system, we want to avoid the natural | In order to produce a grate amount of P4 phage particles that has our death system, we want to avoid the natural | ||
early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the | early lysis that occur when WT P4 and P2 interact. This avoidance will be achieved by taking the control of the | ||
- | two major regulators of P2 morphopoietic genes. The control systems that is going to be implemented is constituted | + | two major regulators of P2 morphopoietic genes and deleting them form lysogenic P2, this will yield a P2 phage |
- | + | which only has capsid and tail genes. The control systems that is going to be implemented is constituted by a | |
- | allow us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence | + | promoter inducible by IPTG in conjunction with transactivators cox and ogr from phage P2. All this will |
- | + | allow us to grow bacteria in grate quantities and induce lysis when ever we want and as a consequence a grate | |
+ | production of modified P2. | ||
** Qualitative characterization of multipromter. | ** Qualitative characterization of multipromter. |
Revision as of 21:51, 21 October 2009