Team:LCG-UNAM-Mexico/Wet Lab/Objectives
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We want P4 to work as a [[Team:LCG-UNAM-Mexico/Description#Delivery|transduction vector for biobricks]]. Several things need to be done, which include individual amplification of P4 sid1 essential region, which we expect to be sufficient for a stable permanence of the biobrick inside the cell. To test this, the first biobrick added will be a reporter gene, which is intended to permanently stay with our P4 version. This is enough to further produce our P4 viral particles and assess functionality of the delivery by transduction into several wild-type bacterial strains as reported in literature. | We want P4 to work as a [[Team:LCG-UNAM-Mexico/Description#Delivery|transduction vector for biobricks]]. Several things need to be done, which include individual amplification of P4 sid1 essential region, which we expect to be sufficient for a stable permanence of the biobrick inside the cell. To test this, the first biobrick added will be a reporter gene, which is intended to permanently stay with our P4 version. This is enough to further produce our P4 viral particles and assess functionality of the delivery by transduction into several wild-type bacterial strains as reported in literature. |
Revision as of 22:24, 21 October 2009