Team:LCG-UNAM-Mexico/Wet Lab/Objectives
From 2009.igem.org
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We want P4 to work as a [[Team:LCG-UNAM-Mexico/Description#Delivery|transduction vector for biobricks]]. Several things need to be done, which include <br>individual amplification of P4 sid1 essential region, which we expect to be sufficient for a stable permanence <br>of the biobrick inside the cell. To test this, the first biobrick added will be a reporter gene, which is intended <br>to permanently stay with our P4 version. This is enough to further produce our P4 viral particles and assess<br> functionality of the delivery by transduction into several wild-type bacterial strains as reported in literature. | We want P4 to work as a [[Team:LCG-UNAM-Mexico/Description#Delivery|transduction vector for biobricks]]. Several things need to be done, which include <br>individual amplification of P4 sid1 essential region, which we expect to be sufficient for a stable permanence <br>of the biobrick inside the cell. To test this, the first biobrick added will be a reporter gene, which is intended <br>to permanently stay with our P4 version. This is enough to further produce our P4 viral particles and assess<br> functionality of the delivery by transduction into several wild-type bacterial strains as reported in literature. | ||
- | **Checking functionality for the Kamikaze system | + | **Checking functionality for the [[Team:LCG-UNAM-Mexico/Description#Defense|Kamikaze system]] |
Now we will be ready to ligate the entire device. Many things have to be assessed, as the complete packaging and <br>delivery of the vector into the host cells, and of course, the functionality of the system. This involves <br>checking for presence and reaction to AHL, production of antisense RNAs and many parameter calculations. | Now we will be ready to ligate the entire device. Many things have to be assessed, as the complete packaging and <br>delivery of the vector into the host cells, and of course, the functionality of the system. This involves <br>checking for presence and reaction to AHL, production of antisense RNAs and many parameter calculations. | ||
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**P2 control system functionality | **P2 control system functionality | ||
- | The cox/ogr control system in which [[Team:LCG-UNAM-Mexico/Wet lab/Objectives#Uriel Urquiza|Uriel]] is working needs experimental validation of functionality.<br>I intend to transform P2 lysogenic strains and expect lysis through activation of such genes.<br>This will prove the construction works. | + | The [[Team:LCG-UNAM-Mexico/Description#P4sid1 standardized production|cox/ogr]] control system in which [[Team:LCG-UNAM-Mexico/Wet lab/Objectives#Uriel Urquiza|Uriel]] is working needs experimental validation of functionality.<br>I intend to transform P2 lysogenic strains and expect lysis through activation of such genes.<br>This will prove the construction works. |
**P4 as an iGEM Plasmid | **P4 as an iGEM Plasmid |
Revision as of 23:06, 21 October 2009