Team:Minnesota/Parts Characterization

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Parts Characterization

We chose five promoters that were included in the 2009 iGEM Kit to characterize. Using part [http://partsregistry.org/wiki/index.php/Part:BBa_F2620 BBa_F2620], which was characterized by a group from MIT in 2004, as a template, we characterized the following parts:

PartDescriptionRegulatorsPeople
[http://partsregistry.org/Part:BBa_I14032 I14032]Constitutive promoter classified as repressibleIPTGPrinceton 2004
[http://partsregistry.org/Part:BBa_J13002 J13002]Two TetR binding sites and RBSaTcUT Austin 2005
[http://partsregistry.org/Part:BBa_I14015 I14015]LasR, 3OC12HSL aTc regulated promoterLasR, 3C12HSL, aTcPrinceton 2004
[http://partsregistry.org/Part:BBa_K091101 K091101]TTL AND gateIPTG, aTcDavidson Missouri-Western 2008
[http://partsregistry.org/Part:BBa_R0011 R0011]Inverting regulatory region controlled by LacIIPTGRegistry