Team:Newcastle/Project/Labwork/OurProtocols

From 2009.igem.org

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* [https://2009.igem.org/Team:Newcastle/Project/Labwork/MoreProtocols/DNAGel DNA Gel Electrophoresis]
* [https://2009.igem.org/Team:Newcastle/Project/Labwork/MoreProtocols/DNAGel DNA Gel Electrophoresis]
Need to analyse some DNA? Read our article for guidelines, hints and tips on how to carry out a successful gel electrophoresis on DNA samples.
Need to analyse some DNA? Read our article for guidelines, hints and tips on how to carry out a successful gel electrophoresis on DNA samples.
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* [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/EthanolPrecip Ethanol Precipitation]
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Want to analyse some DNA? Ethanol still in the sample? Read this protocol for an efficient method of precipitating the ethanol from your DNA sample
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* [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/Spectrophotometer NanoDrop Spectrophotometer]
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Need to meausure the DNA concentrations after doing a midiprep? Read this protocol on how to use spectrometer to mesure your DNA concentrations.
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* [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/TransformBac Transforming into ''Bacillus subtilis 168'']
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Been cloning BioBricks into ''E.coli'' and now want to clone the BioBricks into ''Bacillus subtilis''? Have you got a suitable vector plasmid for transforming into ''B. subtilis''? If the answer to both these questions is yes, read this protocol.
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* [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/Making_Agar_Plates Making Agar Plates]
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Read this protocol to prepare agar plates.
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*[https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/SpeedVac Using the Speed Vac]
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Need to dry off some solvent from some DNA plasmid samples? Follow this step by step guide on how to use the speed vac.
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*[https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/PCR_Products Using The PCR Products]
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From extraction of PCR products to transformation with ''E. coli''
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Latest revision as of 13:30, 8 September 2009

Our Designed Protocols

Team Newcastle - iGem2009 - geldoc 2009-07-27.jpg

Need to analyse some DNA? Read our article for guidelines, hints and tips on how to carry out a successful gel electrophoresis on DNA samples.

Want to analyse some DNA? Ethanol still in the sample? Read this protocol for an efficient method of precipitating the ethanol from your DNA sample

Need to meausure the DNA concentrations after doing a midiprep? Read this protocol on how to use spectrometer to mesure your DNA concentrations.

Been cloning BioBricks into E.coli and now want to clone the BioBricks into Bacillus subtilis? Have you got a suitable vector plasmid for transforming into B. subtilis? If the answer to both these questions is yes, read this protocol.

Read this protocol to prepare agar plates.

Need to dry off some solvent from some DNA plasmid samples? Follow this step by step guide on how to use the speed vac.

From extraction of PCR products to transformation with E. coli



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