Team:TUDelft/Achievements

(Difference between revisions)

Revision as of 16:50, 20 October 2009

Verification and Characterization of Wild R751

Verification and Characterization of trbK entry exclusion protein

Design and test of a a method to prevent back propagation of the signal by expressing trbK. This allows having receiver side control over communication.

Verification and Characterization of Conjugation Testing Plasmid 1

A conjugation test was done using our conjugation protocol to verify that Conjugation Testing Plasmid 1 was transmitted during conjugation.

R751 Gene Knockouts

A third attempt to knockout oriTR and trbK is currently in progress using the Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges.

Synthetic Transcriptional Cascade

Assembly of plasmid 1 and 2 ([http://partsregistry.org/Part:BBa_K175046 K175046]) and 2 ([http://partsregistry.org/Part:BBa_K175047 K175047]).
Partial confirmation of sequence of both plasmids.
Transformation in Escherichia coli
Confirmation of both biobrick individual functionality. ... more

Biosynthetic AND gate

Successful assembly of parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175023 K175023] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175024 K175024] which correspond to Plasmid 1 and 2 of the Biosynthetic AND gate section respectively.
Electroporation of plasmid 1 and 2 into Escherichia coli TOP10 cells... more

Lock and Key Library

Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans).
Online lock and key generator derived from the algorithm.
Construction of four DNA sequences in biobrick format and [http://partsregistry.org/wiki/index.php?title=Part:BBa_pSB1A3 pSB1A3] plasmid backbone which encode lock for medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175031 K175031]), key for the lock of medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175032 K175032]), lock for weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175029 K175029]) or key for the lock of weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175030 K175030]).
Assembly of two composed biobricks ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175034 K175034] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175035 K175035]), designed to test the functionality of the lock/key pairs constructed.
Assembly of two GFP generators under the control of PLacI with weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175033 K175033]) or medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175048 K175048])... more

@@ Line 6: / Line 6: @@
 '''''Verification and Characterization of Wild R751'''''
 *Determination of wild R751 conjugation efficiency.
+'''''Verification and Characterization of trbK entry exclusion protein'''''
 *Design and test of a a method to prevent back propagation of the signal by expressing trbK. This allows having receiver side control over communication.
+'''''Verification and Characterization of Conjugation Testing Plasmid 1'''''
+*A conjugation test was done using our conjugation protocol to verify that Conjugation Testing Plasmid 1 was transmitted during conjugation.
+'''''R751 Gene Knockouts'''''
+*A third attempt to knockout oriTR and trbK is currently in progress using the Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges.
 [https://2009.igem.org/Team:TUDelft/Conjugation_Results more]