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Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest
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(New page: {{:Team:Todai-Tokyo/Template}} == Restriction Enzyme Digest Protocol == 1. Mix the following in a eppendorf tube: * Xµl plasmid DNA （to a final concentration of 300ng/30ul） * 3...) |
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* (1.5µl Restriction Enzyme 2, if applicable) | * (1.5µl Restriction Enzyme 2, if applicable) | ||
* MilliQ up to 30ul | * MilliQ up to 30ul | ||
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2. Incubate at 37ºC for 1h or more | 2. Incubate at 37ºC for 1h or more | ||
Revision as of 07:32, 20 October 2009
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Restriction Enzyme Digest Protocol
1. Mix the following in a eppendorf tube:
- Xµl plasmid DNA （to a final concentration of 300ng/30ul）
- 3µl 10 H buffer
- 1.5µl Restriction Enzyme 1
- (1.5µl Restriction Enzyme 2, if applicable)
- MilliQ up to 30ul
2. Incubate at 37ºC for 1h or more
