Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest

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Restriction Enzyme Digest Protocol

1. Mix the following in a eppendorf tube:

  • Xµl plasmid DNA (to a final concentration of 300ng/30ul)
  • 3µl 10 H buffer
  • 1.5µl Restriction Enzyme 1
  • (1.5µl Restriction Enzyme 2, if applicable)
  • MilliQ up to 30ul

2. Incubate at 37ºC for 1h or more