Team:UCSF/Notebook

From 2009.igem.org

(Difference between revisions)
(HL60 Group)
(Dictyostelium Group)
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*[[Allen Cai's notebook]]:  
*[[Allen Cai's notebook]]:  
*[[Alex Smith's notebook]]:
*[[Alex Smith's notebook]]:
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*[[Edna Miao's notebook]]: Transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.
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*[[Edna Miao's notebook]]: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells.  
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells.  
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.

Revision as of 03:52, 13 October 2009

HL60 Group

  • Goals:
  • Engineering Navigation:
  • sending cells to new targets - GPCR Screening
  • tuning receptor sensitivity - Forced localization of effector proteins to GPCRs
  • Payload:
  • can we make our cells carry stuff? - attach beads to cells

>

Dictyostelium Group

  • Goals:
  • Engineering brakes and accelerators:
  • modulating PIP3 polarity by building synthetic protein based feedback loops
  • workflow:
  • 1. PCR parts: catalytic and localization domains
  • 2. Create combinations of localization domains and catalytic domains
  • 3. Generate Dictyostelium strains expressing feedback elements
  • 4. Measure motility parameters (speed, directionality)
  • 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?

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