Team:UNIPV-Pavia/Freezer

From 2009.igem.org

(Difference between revisions)

Latest revision as of 12:32, 21 October 2009

Biobrick/ Ligation Name	Description	Creation Date	Strain	Vector	Quality Control
J23100(J100) replaced by "J23100 Jun4"	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23101(J101) replaced by "J23101 Jun4"	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23102(J102)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23103(J103)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23104(J104)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23105(J105)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23106(J106)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23110(J110)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23112(J112)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23113(J113)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23114(J114)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23116(J116)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23117(J117)	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118) replaced by "J23118 Jun4"	constitutive promoter	May 25	TOP10	J61002(Amp)	sequence ok (MIT)
E0240(GFP3)	GFP protein generator (RBS-GFP-TT)	May 25	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
K131009(E2)	colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)	May 28	TOP10	pSB1AK3	sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB)	colE7 lysis gene	May 28	TOP10	pSB1A2	sequence ok (MIT)
K124017(lysis21)	Phage Lysis Cassette	May 28	TOP10	pSB1AK3	sequence BAD (MIT)
B0015(TT)	Double terminator	May 28	TOP10	pSB1AK3	sequence ok (MIT)
I732017 (RBS-lacZ)	RBS-lacZ	May 28	TOP10	pSB1A2	long part (MIT)
B0030	RBS 0.6	Jun 4	TOP10	pSB1A2	sequence ok (MIT,BMR)
B0031	RBS 0.07	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
B0032	RBS 0.3	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
B0033	RBS 0.01	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
B0034	RBS 1	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
P0440(P04)	RBS-tetR-TT	Jun 4	TOP10	pSB1A2	length ok (PCR) sequence ok (MIT)
R0040(Ptet)	Ptet	Jun 4	TOP10	pSB1A2	sequence ok (MIT)
T9002	Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT	Jun 4	TOP10	pSB1A3	sequence BAD (MIT) - sequence ok (BMR)
J23100(J100 Jun4)	constitutive promoter	Jun 4	TOP10	J61002(Amp)	sequence ok (MIT,BMR)
J23101(J101 Jun4)	constitutive promoter	Jun 4	TOP10	J61002(Amp)	sequence ok (MIT)
J23118(J118 Jun4)	constitutive promoter	Jun 4	TOP10	J61002(Amp)	sequence ok (MIT)
A1 (BBa_K173000)	J23100(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	TOP10	pSB1A2	length ok (PCR)
A2 (BBa_K173001)	J23101(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 11	TOP10	pSB1A2	length ok (PCR)
A3-1 (BBa_K173004)	I732017-B0015 (RBS-lacZ-TT)	Jun 12	TOP10	pSB1AK3	length ok (digestion PstI) sequence ok -long part- (BMR)
A4 (BBa_K173005)	P0440-R0040 (RBS-tetR-TT-Ptet)	Jun 11	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A5 (BBa_K173006)	A4-A3 (RBS-tetR-TT-Ptet-RBS-lacZ-TT)	Jun 19	TOP10	pSB1AK3	sequence ok -long part- (BMR)
A6 (BBa_K173007)	J23100(Jun 4)-A4 (Pcon-RBS-tetR-TT-Ptet)	Jun 19	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
BOL1	J23118-B0034-C0012-B0015 (Pcon-RBS-lacI-TT)	Jun 23	TOP10	pSB1A2	sequence ok (BMR)
A7 (BBa_K173002)	J23118(Jun 4)-E0240 (Pcon-RBS-GFP-TT)	Jun 25	TOP10	pSB1A2	length ok (PCR)
R0011(Plac)	Plac	Jun 25	TOP10	pSB1A2	sequence ok (MIT)
K112808(ENTERO)	holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)	Jun 25	TOP10	pSB1A2	sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010).
A8	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	TOP10	pSB1A2	NON PURE PLASMID! (digestion E-P) but works
A9	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jun 29	TOP10	pSB1A2	NON PURE PLASMID! (digestion E-P) but works
A10 thrown away	K117000-B0015 (celB-TT)	Jun 30	TOP10	pSB1AK3	sequence BAD (BMR)
A8pg (BBa_K173008)	A4-E0240 (RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	TOP10	pSB1A2	length ok (digestion E-P)
A9pg (BBa_K173009)	A6-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Jul 3	TOP10	pSB1A2	length ok (digestion E-P)
A11-1 thrown away and replaced	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	TOP10	pSB1A2	length ok (PCR) sequence MUTATED
A11-3 thrown away and replaced	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	TOP10	pSB1A2	length ok (PCR)
A11-6 thrown away and replaced	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Jul 10	TOP10	pSB1A2	length ok (PCR)
F2620 thrown away	Ptet-RBS-luxR-TT-Plux	Jul 10	TOP10	pSB1A2	sequence BAD (MIT, BMR)
A12-2 (BBa_K173011)	J23118(Jun 4)-A4	Jul 16	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A12-3 it is a backup of A12-2	J23118(Jun 4)-A4	Jul 16	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A14-4 thrown away	A11-E0240	Jul 16	TOP10	pSB1A2	NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
A14pg thrown away	A11-E0240	Jul 16	TOP10	pSB1A2	NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
K116001 (PnhaA)	PnhaA-RBS-GFP-TT	Jul 29	NEB 10-beta	pSB1A2	sequence WRONG! it is actually K116002!!!
K116002 (PnhaA+GFP)	PnhaA-RBS-GFP-TT	Jul 29	NEB 10-beta	pSB1A2	sequence WRONG! it is actually J33204!!!
K112405 (Pcad)	cadAB promoter	Jul 29	NEB 10-beta	K112951(Amp+Cm)	sequence ok (BMR)
P0412	RBS-lacI-TT	Jul 29	DH5alpha	pSB1A2	sequence ok (BMR)
I746902	GFPmut3+his tag generator under Pbad	Jul 29	NEB 10-beta	pSB1AK3	sequence ok -long part- (BMR)
I746903	GFPmut3+his tag generator under PT7	Jul 29	NEB 10-beta	pSB1AK3	sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same
K101017 to throw away!	MioC promoter	Jul 29	NEB 10-beta	pSB1A2	sequence BAD (BMR)
F2620MIT1	Ptet-RBS-luxR-TT-Plux	Jul 29	D1210(lacIq)	pSB1A2	sequence ok (BMR)
F2620MIT2	Ptet-RBS-luxR-TT-Plux	Jul 29	D1210(lacIq)	pSB1A2	sequence ok (BMR)
MRGENE1 (BBa_K173016)	pdc	Jul 17	TOP10	pMK-RQ (Kan)	antibiotic ok length ok (digestion X-P) sequence ok (Mr Gene)
MRGENE1-2	pdc	Jul 17	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE1-3	pdc	Jul 17	TOP10	pMK-RQ (Kan)	antibiotic ok sequence ok (Mr Gene)
MRGENE2 (BBa_K173017)	adhB	Jul 17	TOP10	pMA (Amp)	antibiotic ok length ok (digestion X-P) sequence ok (Mr Gene)
MRGENE2-2	adhB	Jul 17	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
MRGENE2-3	adhB	Jul 17	TOP10	pMA (Amp)	antibiotic ok sequence ok (Mr Gene)
B1-13 (BBa_K173013)	B0030-MRGENE1 (RBS-pdc)	Jul 28	TOP10	pSB1A2	length ok (digestion E-P , digestion S-P and digestion E-S) sequence ok (BMR)
B2-5 (BBa_K173014)	B0030-MRGENE2 (RBS-adhB)	Jul 23	TOP10	pSB1A2	length ok (PCR and digestion S-P) sequence ok (BMR)
B3-5 (BBa_K173018)	B1-B0015 (RBS-pdc-TT)	Jul 31	TOP10	pSB1AK3	length ok+extra band (digestion XhoI-P and digestion X-P) sequence ok (BMR)
B4-2 (BBa_K173019)	B2-B0015 (RBS-adhB-TT)	Jul 31	TOP10	pSB1AK3	length ok (digestion XhoI-P) sequence ok (BMR)
B5-3	B1-B4 (RBS-pdc-RBS-adhB-TT)	Aug 12	TOP10	pSB1AK3	length ok+2 extra bands (digestion E-P) sequence ok (BMR)
B6-3 (BBa_K173020)	B2-B3 (RBS-adhB-RBS-pdc-TT)	Aug 12	TOP10	pSB1AK3	length ok+2 extra bands (digestion E-P) sequence ok (BMR)
A11-2 thrown away	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Aug 13	TOP10	pSB1A2	length ok (digestion S-P) sequence MUTATED (BMR)
A11-3 (BBa_K173010)	BOL1-R0011 (Pcon-RBS-lacI-TT-Plac)	Aug 13	TOP10	pSB1A2	length ok (digestion S-P) sequence ok (BMR)
A15-1 it is a backup copy of A15-3	F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 14	TOP10	pSB1A2	length ok (digestion) sequence ok (BMR)
A15-2 thrown away	F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 14	TOP10	pSB1A2	length ok (digestion) sequence WRONG (BMR)
A15-3 (BBa_K173015)	F2620MIT1-K112808 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 14	TOP10	pSB1A2	length ok (digestion) sequence ok (BMR)
B8-5 thrown away	A4-B6 (RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)	Aug 21	TOP10	pSB1AK3	length ??? (digestion E-P) sequence WRONG (BMR)
A14-3 to throw away!	A11(-2 Aug13)-E0240 (Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)	Aug 21	TOP10	pSB1A2	length ok (PCR) sequence of A11-2(Aug13) was MUTATED (BMR)
A16-4 (BBa_K173026)	A12-E0240 (Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Aug 21	TOP10	pSB1A2	length ok (PCR) sequence ok (BMR)
A17-4 to throw away!	R0011-E0240 (Plac-RBS-GFP-TT)	Aug 28	TOP10	pSB1A2	length ok (PCR) sequence WRONG! ligation failed.
A18 thrown away	A15(-2)-pSB3K3 (Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)	Aug 28	TOP10	pSB3K3	not screened, its parent plasmid (A15-2) was wrong
K116002 (PnhaA+GFP)	PnhaA-RBS-GFP-TT	Sep 4	TOP10	pSB1A2	sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter
B5new2-3 (BBa_K173003)	B1-B4 (RBS-pdc-RBS-adhB-TT)	Sep 1	TOP10	pSB1AK3	length ok (digestion E-P), sequence ok -long part- (BMR)
B9-2 (BBa_K173021)	F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)	Sep 4	TOP10	pSB1AK3	length ok (digestion E-P), sequence ok -long part- (BMR)
B10-2 (BBa_K173022)	F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT)	Sep 4	TOP10	pSB1AK3	length ok + 2 extra bands (digestion E-P), sequence ok -long part- (BMR)
B9new-1 to 5	F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)	Oct 1	TOP10	pSB1AK3	length ok (digestion E-P)
A19	F2620	Oct 1	TOP10	pSB4C5	length ok (digestion E-P)
A21	A19-B5new2(-3)	Oct 8	TOP10	pSB4C5	length ok (digestion E-P)
A14S-3 (BBa_K173012)	J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240)	?	TOP10	pSB1A2	length ok (digestion E-P)
A17new (BBa_K173025)	Plac-RBS-GFP-TT (R0011-E0240)	Sep 4	TOP10	pSB1A2	length ok (PCR), sequence ok (BMR)
A20	F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT)	Oct 8	TOP10	pSB1A2	length ok (digestion E-P)
I52002 (4C5)	plasmid	?	TOP10	pSB4C5	length ok (digestion E-P)
I714891 (3K3)	plasmid	Aug 21	TOP10	pSB3K3	length ok (digestion E-P)
B14 (BBa_K173023)	F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT)	Oct 16	TOP10	pSB1A2	to screen
B15 (BBa_K173024)	F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT)	Oct 16	TOP10	pSB1A2	to screen

@@ Line 1: / Line 1: @@
-{{UNIPV-Pavia/StyleCss}}
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-<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
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 __NOTOC__
@@ Line 37: / Line 33: @@
 		<td align="center"  width="165px" valign="center"><font face="papyrus" color="royalblue"><b>Description</td>
                    <td align="center" width="73px" valign="center"><font face="papyrus" color="royalblue"><b>Creation Date</td>
-		<td align="center" width="51px" valign="center"><font face="papyrus" color="royalblue"><b>N° usage</td>
 		<td align="center" width="60px" valign="center"><font face="papyrus" color="royalblue"><b>Strain</td>
 		<td align="center" width="97px" valign="center"><font face="papyrus" color="royalblue"><b>Vector</td>
@@ Line 45: / Line 40: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 53: / Line 47: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 61: / Line 54: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 69: / Line 61: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 77: / Line 68: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 85: / Line 75: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 93: / Line 82: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 101: / Line 89: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 109: / Line 96: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 117: / Line 103: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 125: / Line 110: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 133: / Line 117: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 141: / Line 124: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 149: / Line 131: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">May 25</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 157: / Line 138: @@
 		<td class="prova">GFP protein generator<br>(RBS-GFP-TT)</td>
                  <td class="prova">May 25</td>
-		<td class="prova">5</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 165: / Line 145: @@
 		<td class="prova">colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)</td>
                  <td class="prova">May 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 173: / Line 152: @@
 		<td class="prova">colE7 lysis gene</td>
                  <td class="prova">May 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 181: / Line 159: @@
 		<td class="prova">Phage Lysis Cassette</td>
                  <td class="prova">May 28</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 189: / Line 166: @@
 		<td class="prova">Double terminator</td>
                  <td class="prova">May 28</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
@@ Line 197: / Line 173: @@
 		<td class="prova">RBS-lacZ</td>
                  <td class="prova">May 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 205: / Line 180: @@
 		<td class="prova">RBS 0.6</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 213: / Line 187: @@
 		<td class="prova">RBS 0.07</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 221: / Line 194: @@
 		<td class="prova">RBS 0.3</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 229: / Line 201: @@
 		<td class="prova">RBS 0.01</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 237: / Line 208: @@
 		<td class="prova">RBS 1</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 245: / Line 215: @@
 		<td class="prova">RBS-tetR-TT</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 253: / Line 222: @@
 		<td class="prova">Ptet</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 261: / Line 229: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A3</td>
@@ Line 269: / Line 236: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 277: / Line 243: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
@@ Line 285: / Line 250: @@
 		<td class="prova">constitutive promoter</td>
                  <td class="prova">Jun 4</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">J61002(Amp)</td>
 		<td class="prova">sequence ok (MIT)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A1</td>
+	  <tr class="in_freezer"><td class="prova">A1 (BBa_K173000)</td>
 		<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A2</td>
+	  <tr class="in_freezer"><td class="prova">A2 (BBa_K173001)</td>
 		<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A3-1</td>
+	  <tr class="in_freezer"><td class="prova">A3-1 (BBa_K173004)</td>
 		<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td>
                  <td class="prova">Jun 12</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion)</a> sequence ok -long part- (BMR)</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion PstI)</a> sequence ok -long part- (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A4</td>
+	  <tr class="in_freezer"><td class="prova">A4 (BBa_K173005)</td>
 		<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td>
                  <td class="prova">Jun 11</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a> sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A5</td>
+	  <tr class="in_freezer"><td class="prova">A5 (BBa_K173006)</td>
 		<td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td>
                  <td class="prova">Jun 19</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
 		<td class="prova">sequence ok -long part- (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A6</td>
+	  <tr class="in_freezer"><td class="prova">A6 (BBa_K173007)</td>
 		<td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td>
                  <td class="prova">Jun 19</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 341: / Line 299: @@
 		<td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td>
                  <td class="prova">Jun 23</td>
-		<td class="prova">3</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A7</td>
+	  <tr class="in_freezer"><td class="prova">A7 (BBa_K173002)</td>
 		<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 357: / Line 313: @@
 		<td class="prova">Plac</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
@@ Line 365: / Line 320: @@
 		<td class="prova">holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)</td>
                  <td class="prova">Jun 25</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong terminator at the end of the brick (that is actually K112710, not B0010.</td>
+		<td class="prova">sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010).</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">A8</td>
 		<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jun 29</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion)</a> but works</td>
+		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion E-P)</a> but works</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">A9</td>
 		<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jun 29</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion)</a> but works</td>
+		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion E-P)</a> but works</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">A10<br>thrown away</td>
 		<td class="prova">K117000-B0015<br>(celB-TT)</td>
                  <td class="prova">Jun 30</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
-		<td class="prova">sequence BAD (BMR) - repeat ligation</td>
+		<td class="prova">sequence BAD (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A8pg</td>
+	  <tr class="in_freezer"><td class="prova">A8pg (BBa_K173008)</td>
 		<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jul 3</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion)</a></td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A9pg</td>
+	  <tr class="in_freezer"><td class="prova">A9pg (BBa_K173009)</td>
 		<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                  <td class="prova">Jul 3</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion)</a></td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A11-1<br>to throw away!</td>
+	  <tr class="in_freezer"><td class="prova">A11-1<br>thrown away and replaced</td>
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a><br>sequence MUTATED</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A11-3<br>to throw away!</td>
+	  <tr class="in_freezer"><td class="prova">A11-3<br>thrown away and replaced</td>
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A11-6<br>to throw away!</td>
+	  <tr class="in_freezer"><td class="prova">A11-6<br>thrown away and replaced</td>
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">F2620<br>to throw away!</td>
+	  <tr class="in_freezer"><td class="prova">F2620<br>thrown away</td>
 		<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                  <td class="prova">Jul 10</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">sequence BAD (MIT, BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A12-2</td>
+	  <tr class="in_freezer"><td class="prova">A12-2 (BBa_K173011)</td>
 		<td class="prova">J23118(Jun 4)-A4<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A12-3</td>
+	  <tr class="in_freezer"><td class="prova">A12-3<br>it is a backup of A12-2</td>
 		<td class="prova">J23118(Jun 4)-A4<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A14-4<br>to throw away!</td>
+	  <tr class="in_freezer"><td class="prova">A14-4<br>thrown away</td>
 		<td class="prova">A11-E0240<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a></td>
+		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A14pg<br>to throw away!</td>
+	  <tr class="in_freezer"><td class="prova">A14pg <br>thrown away</td>
 		<td class="prova">A11-E0240<br></td>
                  <td class="prova">Jul 16</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a></td>
+		<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">K116001 (PnhaA)</td>
+		<td class="prova">PnhaA-RBS-GFP-TT<br></td>
+                <td class="prova">Jul 29</td>
+		<td class="prova">NEB 10-beta</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">sequence WRONG! it is actually K116002!!!</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">K116002 (PnhaA+GFP)</td>
 		<td class="prova">PnhaA-RBS-GFP-TT<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
+		<td class="prova">NEB 10-beta</td>
-		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter</td>
+		<td class="prova">sequence WRONG! it is actually J33204!!!</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">K112405 (Pcad)</td>
 		<td class="prova">cadAB promoter<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
+		<td class="prova">NEB 10-beta</td>
-		<td class="prova">?</td>
 		<td class="prova">K112951(Amp+Cm)</td>
 		<td class="prova">sequence ok (BMR)</td>
@@ Line 493: / Line 439: @@
 		<td class="prova">RBS-lacI-TT<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
+		<td class="prova">DH5alpha</td>
-		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">sequence ok (BMR)</td>
@@ Line 501: / Line 446: @@
 		<td class="prova">GFPmut3+his tag generator under Pbad<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
+		<td class="prova">NEB 10-beta</td>
-		<td class="prova">?</td>
 		<td class="prova">pSB1AK3</td>
 		<td class="prova">sequence ok -long part- (BMR)</td>
@@ Line 509: / Line 453: @@
 		<td class="prova">GFPmut3+his tag generator under PT7<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
+		<td class="prova">NEB 10-beta</td>
-		<td class="prova">?</td>
 		<td class="prova">pSB1AK3</td>
 		<td class="prova">sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same</td>
@@ Line 517: / Line 460: @@
 		<td class="prova">MioC promoter<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
+		<td class="prova">NEB 10-beta</td>
-		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">sequence BAD (BMR)</td>
@@ Line 525: / Line 467: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">1</td>
+		<td class="prova">D1210(lacIq)</td>
-		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">sequence ok (BMR)</td>
@@ Line 533: / Line 474: @@
 		<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                  <td class="prova">Jul 29</td>
-		<td class="prova">0</td>
+		<td class="prova">D1210(lacIq)</td>
-		<td class="prova">?</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">MRGENE1</td>
+	  <tr class="in_freezer"><td class="prova">MRGENE1 (BBa_K173016)</td>
 		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMK-RQ<br>(Kan)</td>
-		<td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_Digestion_mrGene_B0030.jpg">(digestion)</a><br>sequence ok (Mr Gene)</td>
+		<td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_Digestion_mrGene_B0030.jpg">(digestion X-P)</a><br>sequence ok (Mr Gene)</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE1-2</td>
 		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMK-RQ<br>(Kan)</td>
@@ Line 557: / Line 495: @@
 		<td class="prova">pdc</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMK-RQ<br>(Kan)</td>
 		<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">MRGENE2</td>
+	  <tr class="in_freezer"><td class="prova">MRGENE2 (BBa_K173017)</td>
 		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMA<br>(Amp)</td>
-		<td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_Digestion_mrGene_B0030.jpg">(digestion)</a><br>sequence ok (Mr Gene)</td>
+		<td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_Digestion_mrGene_B0030.jpg">(digestion X-P)</a><br>sequence ok (Mr Gene)</td>
              </tr>
 	  <tr class="in_freezer"><td class="prova">MRGENE2-2</td>
 		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMA<br>(Amp)</td>
@@ Line 581: / Line 516: @@
 		<td class="prova">adhB</td>
                  <td class="prova">Jul 17</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pMA<br>(Amp)</td>
 		<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">B1-13</td>
+	  <tr class="in_freezer"><td class="prova">B1-13 (BBa_K173013)</td>
 		<td class="prova">B0030-MRGENE1<br>(RBS-pdc)</td>
                  <td class="prova">Jul 28</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion E-P</a><br>, <a href="https://2009.igem.org/Image:Pv_digestion_B1_S-P.jpg">digestion S-P</a> and <a href="https://2009.igem.org/Image:Pv_B1E-S_B2E-S_B0015E-X_cut.jpg">digestion E-S</a>)<br>sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">B2-5</td>
+	  <tr class="in_freezer"><td class="prova">B2-5 (BBa_K173014)</td>
 		<td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td>
                  <td class="prova">Jul 23</td>
-		<td class="prova">2</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_B2pcr_A14pgPstI.jpg">PCR</a><br> and <a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion S-P</a>)<br>sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">B3-5</td>
+	  <tr class="in_freezer"><td class="prova">B3-5 (BBa_K173018)</td>
 		<td class="prova">B1-B0015<br>(RBS-pdc-TT)</td>
                  <td class="prova">Jul 31</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
-		<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_.jpg">digestion XhoI-P</a><br> and <a href="https://2009.igem.org/Image:Pv_.jpg">digestion X-P</a>)<br>sequence ok (BMR)</td>
+		<td class="prova">length ok+extra band (<a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">digestion XhoI-P</a><br> and <a href="https://2009.igem.org/Image:Pv_B3_digestion_X-P.jpg">digestion X-P</a>)<br>sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">B4-2</td>
+	  <tr class="in_freezer"><td class="prova">B4-2 (BBa_K173019)</td>
 		<td class="prova">B2-B0015<br>(RBS-adhB-TT)</td>
                  <td class="prova">Jul 31</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion XhoI-P)</a><br>sequence ok (BMR)</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">(digestion XhoI-P)</a><br>sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A11-2<br>to throw away!</td>
+	  <tr class="in_freezer"><td class="prova">B5-3</td>
+		<td class="prova">B1-B4<br>(RBS-pdc-RBS-adhB-TT)</td>
+                <td class="prova">Aug 12</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1AK3</td>
+		<td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B6-3 (BBa_K173020)</td>
+		<td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td>
+                <td class="prova">Aug 12</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1AK3</td>
+		<td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A11-2<br>thrown away</td>
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Aug 13</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_.jpg">digestion</a> sequence MUTATED (BMR)</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence MUTATED (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A11-3</td>
+	  <tr class="in_freezer"><td class="prova">A11-3 (BBa_K173010)</td>
 		<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                  <td class="prova">Aug 13</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_.jpg">digestion</a> sequence ok (BMR)</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A15-1</td>
+	  <tr class="in_freezer"><td class="prova">A15-1<br>it is a backup copy of A15-3</td>
 		<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                  <td class="prova">Aug 14</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A15-2</td>
+	  <tr class="in_freezer"><td class="prova">A15-2<br>thrown away</td>
 		<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                  <td class="prova">Aug 14</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a></td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence WRONG (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">A15-3</td>
+	  <tr class="in_freezer"><td class="prova">A15-3 (BBa_K173015)</td>
 		<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                  <td class="prova">Aug 14</td>
-		<td class="prova">0</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1A2</td>
 		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">B5-3</td>
+	  <tr class="in_freezer"><td class="prova">B8-5<br>thrown away</td>
-		<td class="prova">B1-B4<br>(RBS-pdc-RBS-adhB-TT)</td>
+		<td class="prova">A4-B6<br>(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)</td>
-                 <td class="prova">Jul 31</td>
+                 <td class="prova">Aug 21</td>
-		<td class="prova">1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion XhoI-P)</a><br>sequence ok (BMR)</td>
+		<td class="prova">length ??? (digestion E-P) sequence WRONG (BMR)</td>
              </tr>
-	  <tr class="in_freezer"><td class="prova">B6-3</td>
+	  <tr class="in_freezer"><td class="prova">A14-3<br>to throw away!</td>
-		<td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td>
+		<td class="prova">A11(-2 Aug13)-E0240<br>(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)</td>
-                 <td class="prova">Jul 31</td>
+                 <td class="prova">Aug 21</td>
-		<td class="prova">1</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence of A11-2(Aug13) was MUTATED (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A16-4 (BBa_K173026)</td>
+		<td class="prova">A12-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
+                <td class="prova">Aug 21</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence ok (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A17-4<br>to throw away!</td>
+		<td class="prova">R0011-E0240<br>(Plac-RBS-GFP-TT)</td>
+                <td class="prova">Aug 28</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence WRONG! ligation failed.</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A18<br>thrown away</td>
+		<td class="prova">A15(-2)-pSB3K3<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
+                <td class="prova">Aug 28</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB3K3</td>
+		<td class="prova">not screened, its parent plasmid (A15-2) was wrong</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">K116002 (PnhaA+GFP)</td>
+		<td class="prova">PnhaA-RBS-GFP-TT<br></td>
+                <td class="prova">Sep 4</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B5new2-3 (BBa_K173003)</td>
+		<td class="prova">B1-B4 (RBS-pdc-RBS-adhB-TT)</td>
+                <td class="prova">Sep 1</td>
 		<td class="prova">TOP10</td>
 		<td class="prova">pSB1AK3</td>
-		<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion XhoI-P)</a><br>sequence ok (BMR)</td>
+		<td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B9-2 (BBa_K173021)</td>
+		<td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td>
+                <td class="prova">Sep 4</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1AK3</td>
+		<td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B10-2 (BBa_K173022)</td>
+		<td class="prova">F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT)</td>
+                <td class="prova">Sep 4</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1AK3</td>
+		<td class="prova">length ok + 2 extra bands (digestion E-P), sequence ok -long part- (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B9new-1 to 5</td>
+		<td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td>
+                <td class="prova">Oct 1</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1AK3</td>
+		<td class="prova">length ok (digestion E-P)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A19</td>
+		<td class="prova">F2620</td>
+                <td class="prova">Oct 1</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB4C5</td>
+		<td class="prova">length ok (digestion E-P)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A21</td>
+		<td class="prova">A19-B5new2(-3)</td>
+                <td class="prova">Oct 8</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB4C5</td>
+		<td class="prova">length ok (digestion E-P)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A14S-3 (BBa_K173012)</td>
+		<td class="prova">J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240)</td>
+                <td class="prova">?</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">length ok (digestion E-P)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A17new (BBa_K173025)</td>
+		<td class="prova">Plac-RBS-GFP-TT (R0011-E0240)</td>
+                <td class="prova">Sep 4</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">length ok (PCR), sequence ok (BMR)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">A20</td>
+		<td class="prova">F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
+                <td class="prova">Oct 8</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">length ok (digestion E-P)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">I52002 (4C5)</td>
+		<td class="prova">plasmid</td>
+                <td class="prova">?</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB4C5</td>
+		<td class="prova">length ok (digestion E-P)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">I714891 (3K3)</td>
+		<td class="prova">plasmid</td>
+                <td class="prova">Aug 21</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB3K3</td>
+		<td class="prova">length ok (digestion E-P)</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B14 (BBa_K173023)</td>
+		<td class="prova">F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT)</td>
+                <td class="prova">Oct 16</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">to screen</td>
+            </tr>
+	  <tr class="in_freezer"><td class="prova">B15 (BBa_K173024)</td>
+		<td class="prova">F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT)</td>
+                <td class="prova">Oct 16</td>
+		<td class="prova">TOP10</td>
+		<td class="prova">pSB1A2</td>
+		<td class="prova">to screen</td>
              </tr>