Team:Wash U/Protocol
From 2009.igem.org
(Difference between revisions)
Line 16: | Line 16: | ||
*15g Agar | *15g Agar | ||
*up to 1 Liter H20 | *up to 1 Liter H20 | ||
- | + | '''Super Optimal Broth (SOB)''' | |
+ | * 2% w/v bacto-tryptone (20g) | ||
+ | * 0.5% w/v bacto-yeast extract (5g) | ||
+ | * 8.56mM NaCl (0.5g) or 10mM NaCl (0.584g) | ||
+ | * 2.5mM KCl (0.186g) | ||
+ | * ddH2O to 1L | ||
+ | '''Super Optimal Broth with Catabolite repression (SOC)''' | ||
+ | In addition to all of the SOB components, | ||
+ | * | ||
{{WashUbottom}} | {{WashUbottom}} |
Revision as of 17:53, 29 June 2009