Team:Wash U/Protocol

From 2009.igem.org

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'''Polymerase Chain Reaction (PCR)'''
'''Polymerase Chain Reaction (PCR)'''
 +
'''Transformations'''
'''Transformations'''
 +
'''Construction of Biobricks'''
'''Construction of Biobricks'''
 +
'''Gel Electrophoresis'''
'''Gel Electrophoresis'''

Revision as of 17:56, 29 June 2009

Procedures

Polymerase Chain Reaction (PCR)


Transformations


Construction of Biobricks


Gel Electrophoresis


Recipes

Lysogeny Broth (LB) Media

  • 10g Tryptone
  • 5g Yeast Extract
  • 10g NaCl
  • 15g Agar
  • up to 1 Liter H20

Super Optimal Broth (SOB)

  • 2% w/v bacto-tryptone (20g)
  • 0.5% w/v bacto-yeast extract (5g)
  • 8.56mM NaCl (0.5g) or 10mM NaCl (0.584g)
  • 2.5mM KCl (0.186g)
  • ddH2O to 1L

Super Optimal Broth with Catabolite repression (SOC)

  • In addition to all of the SOB components,
  • 10mM MgCl2 (0.952g)
  • 20mM glucose (3.603g)