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Team:Wash U/Protocol
From 2009.igem.org
(Difference between revisions)
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*Adjust pH to 7.0-7.2 | *Adjust pH to 7.0-7.2 | ||
'''Super Optimal Broth (SOB)''' | '''Super Optimal Broth (SOB)''' | ||
| - | * 2% w/v bacto-tryptone (20g) | + | *2% w/v bacto-tryptone (20g) |
| - | * 0.5% w/v bacto-yeast extract (5g) | + | *0.5% w/v bacto-yeast extract (5g) |
| - | * 8.56mM NaCl (0.5g) or 10mM NaCl (0.584g) | + | *8.56mM NaCl (0.5g) or 10mM NaCl (0.584g) |
| - | * 2.5mM KCl (0.186g) | + | *2.5mM KCl (0.186g) |
| - | * ddH2O to 1L | + | *ddH2O to 1L |
'''Super Optimal Broth with Catabolite repression (SOC)''' | '''Super Optimal Broth with Catabolite repression (SOC)''' | ||
*In addition to all of the SOB components, | *In addition to all of the SOB components, | ||
*10mM MgCl2 (0.952g) | *10mM MgCl2 (0.952g) | ||
*20mM glucose (3.603g) | *20mM glucose (3.603g) | ||
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| - | |||
| - | |||
| - | |||
'''TAE Buffer''' | '''TAE Buffer''' | ||
| - | + | *242 g Tris base (2-amino-2-hydroxymethyl-propane-1,3-diol) (= 2 mole) | |
| + | *57.1 ml glacial acetic acid (= 100% acetic acid) (57.19 ml = 1 mole) | ||
| + | *100 ml 0.5 M Na2 EDTA (pH 8.0) | ||
| + | *ddH2O to 1L | ||
'''TBE Buffer''' | '''TBE Buffer''' | ||
| - | + | *53 g of Tris base (CAS# 37186) | |
| + | * 27.5 g of boric acid (CAS# 11280) | ||
| + | *20 ml of 0.5 M EDTA (CAS# 60004) (pH 8.0) | ||
'''Tris EDTA (TE) buffer''' | '''Tris EDTA (TE) buffer''' | ||
*750 mL d-H20 | *750 mL d-H20 | ||
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*fill to 1 L | *fill to 1 L | ||
*adjust pH to 8.5 | *adjust pH to 8.5 | ||
| + | '''Gel (for Electrophoresis)''' | ||
| + | *50 mL 1X TAE buffer | ||
| + | *0.8 g agarose | ||
| + | *2.5 microliters EtBr | ||
{{WashUbottom}} | {{WashUbottom}} | ||
Revision as of 21:54, 29 June 2009
