Team:Wash U/Protocol
From 2009.igem.org
(Difference between revisions)
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* 27.5 g of boric acid (CAS# 11280) | * 27.5 g of boric acid (CAS# 11280) | ||
*20 ml of 0.5 M EDTA (CAS# 60004) (pH 8.0) | *20 ml of 0.5 M EDTA (CAS# 60004) (pH 8.0) | ||
- | '''Tris EDTA (TE) buffer''' | + | '''Tris EDTA (TE) buffer 5X 1L''' |
*750 mL d-H20 | *750 mL d-H20 | ||
*242 g Tris base | *242 g Tris base | ||
Line 89: | Line 89: | ||
*fill to 1 L | *fill to 1 L | ||
*adjust pH to 8.5 | *adjust pH to 8.5 | ||
- | '''Gel (for Electrophoresis)''' | + | '''Agarose Gel (for Electrophoresis)''' |
*50 mL 1X TAE buffer | *50 mL 1X TAE buffer | ||
*0.8 g agarose | *0.8 g agarose | ||
*2.5 microliters EtBr (Caution: EtBr is a known carcinogen) | *2.5 microliters EtBr (Caution: EtBr is a known carcinogen) | ||
*Note: Jacob suggested adding 1.0 microliter EtBr to gel and 3.0 microliters to TAE buffer in rig. | *Note: Jacob suggested adding 1.0 microliter EtBr to gel and 3.0 microliters to TAE buffer in rig. | ||
- | + | '''Polyacrylamide Gel (for Electrophoresis)''' | |
{{WashUbottom}} | {{WashUbottom}} |
Revision as of 16:31, 30 June 2009