Team:Wash U/Protocol
From 2009.igem.org
(Difference between revisions)
Line 89: | Line 89: | ||
*fill to 1 L | *fill to 1 L | ||
*adjust pH to 8.5 | *adjust pH to 8.5 | ||
- | '''Agarose Gel (for | + | '''Agarose Gel (for electrophoresis of DNA >100bp)''' |
*50 mL 1X TAE buffer | *50 mL 1X TAE buffer | ||
*0.8 g agarose | *0.8 g agarose | ||
*2.5 microliters EtBr (Caution: EtBr is a known carcinogen) | *2.5 microliters EtBr (Caution: EtBr is a known carcinogen) | ||
*Note: Jacob suggested adding 1.0 microliter EtBr to gel and 3.0 microliters to TAE buffer in rig. | *Note: Jacob suggested adding 1.0 microliter EtBr to gel and 3.0 microliters to TAE buffer in rig. | ||
- | '''Polyacrylamide Gel (for | + | '''Polyacrylamide Gel (for electrophoresis of DNA <100 bp)''' |
{{WashUbottom}} | {{WashUbottom}} |
Revision as of 16:36, 30 June 2009