Contents
Polymerase Chain Reaction (PCR)
Transformation
Mini-Prep
Digestion
Ligation
Gel Electrophoresis
ATCC medium: Van Niel's yeast agar
Super Optimal Broth (SOB)
Super Optimal Broth with Catabolite repression (SOC)
50X TAE Buffer
5X TBE Buffer
Tris EDTA (TE) buffer 5X 1L
Agarose Gel (for electrophoresis of DNA >100bp)