Team:Wash U/Protocol
From 2009.igem.org
(Difference between revisions)
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== '''Procedures''' == | == '''Procedures''' == | ||
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'''Contents''' | '''Contents''' | ||
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:1. [[Team:Wash_U/Protocol#Polymerase_Chain_Reaction_(PCR)|Polymerase Chain Reaction (PCR)]]<br> | :1. [[Team:Wash_U/Protocol#Polymerase_Chain_Reaction_(PCR)|Polymerase Chain Reaction (PCR)]]<br> | ||
:2. [[Team:Wash_U/Protocol#Transformation|Transformation]]<br> | :2. [[Team:Wash_U/Protocol#Transformation|Transformation]]<br> | ||
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=='''Polymerase Chain Reaction (PCR)'''== | =='''Polymerase Chain Reaction (PCR)'''== | ||
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- | : | + | :general description |
+ | '''Materials''' | ||
+ | :list of materials | ||
+ | '''Procedure''' | ||
+ | :list of steps | ||
- | '''Transformation'''<br> | + | =='''Transformation'''==<br> |
:1. Start thawing the competent cells on crushed ice.<br> | :1. Start thawing the competent cells on crushed ice.<br> | ||
:2. Add 50 µL of thawed competent cells and then 1 - 2 µL of the resuspended DNA to the labelled tubes. Make sure to keep the competent cells on ice.<br> | :2. Add 50 µL of thawed competent cells and then 1 - 2 µL of the resuspended DNA to the labelled tubes. Make sure to keep the competent cells on ice.<br> |
Revision as of 15:51, 6 July 2009