Team:Wash U/Protocol
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=='''Transformation'''==<br> | =='''Transformation'''==<br> | ||
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+ | :General description | ||
+ | '''Materials''' | ||
+ | :List of materials | ||
+ | '''Procedures''' | ||
:1. Start thawing the competent cells on crushed ice.<br> | :1. Start thawing the competent cells on crushed ice.<br> | ||
:2. Add 50 µL of thawed competent cells and then 1 - 2 µL of the resuspended DNA to the labelled tubes. Make sure to keep the competent cells on ice.<br> | :2. Add 50 µL of thawed competent cells and then 1 - 2 µL of the resuspended DNA to the labelled tubes. Make sure to keep the competent cells on ice.<br> |
Revision as of 15:53, 6 July 2009