Team:Wash U/Protocol
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=='''Digestion'''== | =='''Digestion'''== | ||
<font size="2"> | <font size="2"> | ||
- | :Note: The procedures from this point forward assume that DNA has been amplified and purified. If this is not the case, please read the Polymerase Chain Reaction procedure for amplification and the Mini-Prep procedure for purification. | + | :Digestion cuts the DNA at very specific sites with restriction enzymes. This procedure is required for various DNA manipulation techniques including gel electrophoresis and BioBrick assembly. Note: The procedures from this point forward assume that DNA has been amplified and purified. If this is not the case, please read the Polymerase Chain Reaction procedure for amplification and the Mini-Prep procedure for purification. |
'''Materials''' | '''Materials''' | ||
:list of materials | :list of materials |
Revision as of 20:15, 8 July 2009