Team:Wash U/Protocol
From 2009.igem.org
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:Transformation is the genetic alteration of a cell synthetically. In this case, plasmids are used to carry desired genes and coding sequences which are then taken up by the bacterial cells. Once in the cells, the plasmids are transcribed and translated into proteins in the same way as the host's genomic DNA. Transformations allow us to easily express things like antibiotic resistance and green florescent protein (GFP) in desired cells. | :Transformation is the genetic alteration of a cell synthetically. In this case, plasmids are used to carry desired genes and coding sequences which are then taken up by the bacterial cells. Once in the cells, the plasmids are transcribed and translated into proteins in the same way as the host's genomic DNA. Transformations allow us to easily express things like antibiotic resistance and green florescent protein (GFP) in desired cells. | ||
'''Materials''' | '''Materials''' | ||
- | + | * dI H20 | |
+ | * Competent cells | ||
+ | * Plasmid DNA (to be taken up by cells) | ||
+ | * SOC media | ||
+ | * LB agar | ||
+ | * Antibiotics (vary depending on DNA used and are in LB plates) | ||
'''Procedures''' | '''Procedures''' | ||
# Start thawing the competent cells on crushed ice. | # Start thawing the competent cells on crushed ice. |
Revision as of 15:59, 9 July 2009