Team:Wash U/Protocol
From 2009.igem.org
(→Gel Electrophoresis) |
(→Gel Electrophoresis) |
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*Power Supply | *Power Supply | ||
'''Procedures''' | '''Procedures''' | ||
- | + | #Scale out agarose for gel (0.8% - 2.0%) | |
+ | #Add agarose powder to appropriately sized glassware | ||
+ | #Add 1X TAE Buffer | ||
+ | #Microwave until boiling | ||
+ | #Allow solution to cool until glassware is comfortable in hand | ||
+ | #Add 2ul ethidium bromide and swirl | ||
+ | #Pour gel solution into level gel tray mounted on the gel pouring dock | ||
+ | #Insert gel comb with appropriate number of wells | ||
+ | #Allow gel to set up for approximately 20 minutes | ||
+ | #Remove gel from pouring dock and submerge just below surface of 1X TAE buffer in gel box | ||
+ | #Carefully remove gel comb | ||
+ | #Load DNA ladder and samples | ||
+ | #Attach gel box electrodes to power supply and apply desired voltage until completion | ||
[[Team:Wash_U/Protocol#Procedures|Back To Top]] | [[Team:Wash_U/Protocol#Procedures|Back To Top]] | ||
Revision as of 21:35, 9 July 2009