Team:Wash U/Protocol
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=='''BioBrick Assembly'''== | =='''BioBrick Assembly'''== | ||
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- | :BioBrick Assembly is a standard protocol for combining two BioBrick parts and positioning them in a destination plasmid. Specific cut sites for standard restriction enzymes are utilized to simplify the protocol and include EcoR1-HF, Xba1, Spe1 and Pst1. To view the full Biobrick Assembly manual, please click [http://ginkgobioworks.com/support/ here]. | + | :BioBrick Assembly is a standard protocol for combining two BioBrick parts and positioning them in a destination plasmid. Specific cut sites for standard restriction enzymes are utilized to simplify the protocol and include EcoR1-HF, Xba1, Spe1 and Pst1. The assembly consists of two major parts: digestion and ligation. Note that BioBrick assembly requires purified, isolated DNA for all parts involved (upstream, downstream and destination plasmid). To view the full Biobrick Assembly manual, please click [http://ginkgobioworks.com/support/ here]. |
'''Materials''' | '''Materials''' | ||
:List of materials | :List of materials |
Revision as of 15:22, 10 July 2009