Team:Wash U/Protocol
From 2009.igem.org
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(→Ligation) |
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# Add 2 uL of 10X T4 DNA Ligase Reaction Buffer to the 200 uL PCR tube. | # Add 2 uL of 10X T4 DNA Ligase Reaction Buffer to the 200 uL PCR tube. | ||
# Add 1 uL DNA Ligase to the PCR tube and flick to ensure the contents are mized. | # Add 1 uL DNA Ligase to the PCR tube and flick to ensure the contents are mized. | ||
- | # Let the mix stand for | + | # Let the mix stand for 1 hour at room temperature before incubating at 80C for 20 minutes (deactivates enzymes). |
# Store the products at -20C until they are needed for a transformation. | # Store the products at -20C until they are needed for a transformation. | ||
[https://2009.igem.org/Team:Wash_U/Protocol Back To Top] | [https://2009.igem.org/Team:Wash_U/Protocol Back To Top] | ||
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=='''Gel Electrophoresis'''== | =='''Gel Electrophoresis'''== | ||
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Revision as of 16:48, 6 August 2009