Team:Washington-Software

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<h1> Abstract </h1>
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<h2> Abstract </h2>
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<h4>'''BioBrick-A-Bot: Lego robot for Automated BioBrick DNA Assembly'''</h4>
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<h4>'''BioBrick-A-Bot: Lego Robot for Automated BioBrick DNA Assembly'''</h4>
[[Image:Robot Close Up.jpg|thumb|right|'''BioBrick-A-Bot''']]
[[Image:Robot Close Up.jpg|thumb|right|'''BioBrick-A-Bot''']]
Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods.  To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick.  The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface).  We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.
Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods.  To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick.  The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface).  We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.
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<h1> Project Goals </h1>
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<h2> Project Goals </h2>
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#Low Cost – Robot cost significantly lower than $10,000. (Actual cost:~$700)
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#Low Cost – Robot cost significantly lower than $10,000, the price of a commercial liquid handling robot. (Actual cost of BioBrick-a-Bot prototype: ~$700)
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#Hardware Platform that is easily accessible  
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#Hardware Platform that is easily accessible.
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#Hardware Design that is easily replicable by other iGEM teams.
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#Hardware Design that is easily replicable by other iGEM teams.
#Software Design  that is robust, plug and play. Can swap modules from other iGEM teams.
#Software Design  that is robust, plug and play. Can swap modules from other iGEM teams.
#Design that is easily extensible, to allow future collaboration with other iGEM teams.
#Design that is easily extensible, to allow future collaboration with other iGEM teams.
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<h1> The Vision </h1>
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<h2> The Vision </h2>
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[[Image:LegoRoboBrick.jpg|310px|left]][[Image:LegoRoboBrick2.jpg|310px|left]][[Image:LegoRoboBricks.jpg|310px|right]]
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[[Image:LegoRoboBrick.jpg|315px|left]][[Image:LegoRoboBrick2.jpg|315px|left]][[Image:LegoRoboBricks.jpg|313px|right]]
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<h2> Acknowledgements </h2>
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We build BioBrick-A-Bot V1.0 as a prototype and provide a [[Team:Washington-Software/Project |proof-of-principle demonstration]] that it worked. V1.0 will however, not have the full range of functionalities of a commercial liquid handling robot. V1.0 will be made up of 2 LegoRoboBrick pieces, ALPHA and PHI, mounted on a chassis called BETA. We will provide construction details and software so that other iGEM team can replicate and customize their own 'bot's.
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Our iGEM project is sponsored by the [http://depts.washington.edu/bioe/ BioEngineering Department] at the [http://www.washington.edu/ University of Washington]
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In our next version, BioBrick-A-Bot V2.0, we will provide 3 LegoRoboBrick pieces, ALPHA, PHI and MU. We will also support user-defined LegoRoboBrick pieces too. We provide the mechanism where other igem teams can [[Team:Washington-Software/Collaboration | colloborate together ]] to replicate, interchange and extend LegoRoboBricks. Many iGEM teams can contribute and share both Hardware Construction and Software Programs for LegoRoboBrick pieces in a Registry, much like the "Registry of Standard Biological Parts". Our vision will be to make the technologies of creating and sharing 'bots' easily available, replacing labour intensive manual work, and cost intensive machines.
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[[Image:bioelogo.jpg]]
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[[Image:uw_logo.jpg]]
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To do: Acknowlegement: BioE
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Latest revision as of 02:25, 22 October 2009

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Contents

Abstract

BioBrick-A-Bot: Lego Robot for Automated BioBrick DNA Assembly

BioBrick-A-Bot

Commercial Liquid Handling Systems are extremely expensive, and are typically beyond the reach of the average molecular biologist interested in performing high throughput methods. To address this problem, we design and implement a liquid handling system built from commonly accessible Legos. Our goal is the automation of BioBrick assembly on a platform that can itself be easily replicated and we demonstrate a proof-of-principle for this system by transferring colored dye solutions on a 96-well plate. We introduce a new concept called LegoRoboBrick. The liquid handling system is build from 3 new LegoRoboBrick modular components: ALPHA (Automated Lego Pipette Head Assembly), BETA (BioBrick Environmental Testing Apparatus), and PHI (Pneumatic Handling Interface). We will demonstrate that the same BioBrick assembly software can run on multiple plug-and-play LegoRoboBrick instances with different physical dimensions and geometric configurations. The modular design of LegoRoboBricks allows easy extension of new laboratory functionalities in the future.

Project Goals

  1. Low Cost – Robot cost significantly lower than $10,000, the price of a commercial liquid handling robot. (Actual cost of BioBrick-a-Bot prototype: ~$700)
  2. Hardware Platform that is easily accessible.
  3. Hardware Design that is easily replicable by other iGEM teams.
  4. Software Design that is robust, plug and play. Can swap modules from other iGEM teams.
  5. Design that is easily extensible, to allow future collaboration with other iGEM teams.

The Vision

LegoRoboBrick.jpg
LegoRoboBrick2.jpg
LegoRoboBricks.jpg

Acknowledgements

Our iGEM project is sponsored by the [http://depts.washington.edu/bioe/ BioEngineering Department] at the [http://www.washington.edu/ University of Washington]

Bioelogo.jpg

Uw logo.jpg