Template:Team:KULeuven/6 September 2009/VanillinProduction

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  • Restriction digest overnight of sam5 (1,2) and sam8 (1,2)
  • Growth of EF Ligation (woohoo!)
    • Transferred 4 colonies from EF ligation to a new plate
    • Put the 4 transferred colonies on liquid medium

A miniprep and restriction digest (ON) of sam5, sam8, ech and fcs was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.